Phosrestin I undergoes the earliest light-induced phosphorylation by a calcium/calmodulin-dependent protein kinase in drosophila photoreceptors

Hiroyuki Matsumoto, Biji T. Kurien, Yuichiro Takagi, Esther S. Kahn, Tomoya Kinumi, Naoka Komori, Takuma Yamada, Fumio Hayashi, Kunio Isono, William L. Pak, Kenneth W. Jackson, Sara L. Tobin

Research output: Contribution to journalArticle

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Abstract

Activation of PI-PLC initiates two independent branches of protein phosphorylation cascades catalyzed by either PKC or Ca2+/calmodulin-dependent protein kinase (CaMK). We find that phosrestin I (PRI), a Drosophila homolog of vertebrate photoreceptor arrestin, undergoes light-induced phosphorylation on a subsecond time scale which is faster than that of any other protein in vivo. We determine that a CaMK activity is responsible for in vitro PRI phosphorylation at Ser366 in the C-terminal tryptic segment, MetLysSer(P)IIeGIuGInHisArg, in which Ser(P) represents phosphoserine366. We also demonstrate that Ser366 is the phosphorylation site of PRI in vivo by identifying the molecular species resulting from in-gel tryptic digestion of purified phospho-PRI using HPLC-electrospray ionization tandem quadrupole mass spectroscopy. From these data, we conclude that the CaMK pathway, not the PKC pathway, is responsible for the earliest protein phosphorylation event following activation of PI-PLC in living Drosophila photoreceptors.

Original languageEnglish (US)
Pages (from-to)997-1010
Number of pages14
JournalNeuron
Volume12
Issue number5
DOIs
StatePublished - 1994
Externally publishedYes

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Calcium-Calmodulin-Dependent Protein Kinases
Phosphorylation
Light
Drosophila
Arrestin
Vertebrate Photoreceptor Cells
Proteins
Digestion
Mass Spectrometry
Gels
High Pressure Liquid Chromatography
Drosophila Caki protein

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Phosrestin I undergoes the earliest light-induced phosphorylation by a calcium/calmodulin-dependent protein kinase in drosophila photoreceptors. / Matsumoto, Hiroyuki; Kurien, Biji T.; Takagi, Yuichiro; Kahn, Esther S.; Kinumi, Tomoya; Komori, Naoka; Yamada, Takuma; Hayashi, Fumio; Isono, Kunio; Pak, William L.; Jackson, Kenneth W.; Tobin, Sara L.

In: Neuron, Vol. 12, No. 5, 1994, p. 997-1010.

Research output: Contribution to journalArticle

Matsumoto, H, Kurien, BT, Takagi, Y, Kahn, ES, Kinumi, T, Komori, N, Yamada, T, Hayashi, F, Isono, K, Pak, WL, Jackson, KW & Tobin, SL 1994, 'Phosrestin I undergoes the earliest light-induced phosphorylation by a calcium/calmodulin-dependent protein kinase in drosophila photoreceptors', Neuron, vol. 12, no. 5, pp. 997-1010. https://doi.org/10.1016/0896-6273(94)90309-3
Matsumoto, Hiroyuki ; Kurien, Biji T. ; Takagi, Yuichiro ; Kahn, Esther S. ; Kinumi, Tomoya ; Komori, Naoka ; Yamada, Takuma ; Hayashi, Fumio ; Isono, Kunio ; Pak, William L. ; Jackson, Kenneth W. ; Tobin, Sara L. / Phosrestin I undergoes the earliest light-induced phosphorylation by a calcium/calmodulin-dependent protein kinase in drosophila photoreceptors. In: Neuron. 1994 ; Vol. 12, No. 5. pp. 997-1010.
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