Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release

T. Kuner, Y. Li, K. R. Gee, L. F. Bonewald, G. J. Augustine

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The time at which the N-ethylmaleimide-sensitive factor (NSF) acts during synaptic vesicle (SV) trafficking was identified by time-controlled perturbation of NSF function with a photoactivatable inhibitory peptide. Photolysis of this caged peptide in the squid giant presynaptic terminal caused an abrupt (0.2 s) slowing of the kinetics of the postsynaptic current (PSC) and a more gradual (2-3 s) reduction in PSC amplitude. Based on the rapid rate of these inhibitory effects relative to the speed of SV recycling, we conclude that NSF functions in reactions that immediately precede neurotransmitter release. Our results indicate the locus of SNARE protein recycling in presynaptic terminals and reveal NSF as a potential target for rapid regulation of transmitter release.

Original languageEnglish (US)
Pages (from-to)347-352
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number1
DOIs
StatePublished - Jan 8 2008

Fingerprint

N-Ethylmaleimide-Sensitive Proteins
Photolysis
Neurotransmitter Agents
Synaptic Potentials
Peptides
Synaptic Vesicles
Presynaptic Terminals
SNARE Proteins
Decapodiformes

Keywords

  • Caged probes
  • Exocytosis
  • Synaptic transmission
  • Synaptic vesicle cycle

ASJC Scopus subject areas

  • General

Cite this

Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release. / Kuner, T.; Li, Y.; Gee, K. R.; Bonewald, L. F.; Augustine, G. J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 1, 08.01.2008, p. 347-352.

Research output: Contribution to journalArticle

@article{66126a8442a543eaafbde5179ef848be,
title = "Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release",
abstract = "The time at which the N-ethylmaleimide-sensitive factor (NSF) acts during synaptic vesicle (SV) trafficking was identified by time-controlled perturbation of NSF function with a photoactivatable inhibitory peptide. Photolysis of this caged peptide in the squid giant presynaptic terminal caused an abrupt (0.2 s) slowing of the kinetics of the postsynaptic current (PSC) and a more gradual (2-3 s) reduction in PSC amplitude. Based on the rapid rate of these inhibitory effects relative to the speed of SV recycling, we conclude that NSF functions in reactions that immediately precede neurotransmitter release. Our results indicate the locus of SNARE protein recycling in presynaptic terminals and reveal NSF as a potential target for rapid regulation of transmitter release.",
keywords = "Caged probes, Exocytosis, Synaptic transmission, Synaptic vesicle cycle",
author = "T. Kuner and Y. Li and Gee, {K. R.} and Bonewald, {L. F.} and Augustine, {G. J.}",
year = "2008",
month = "1",
day = "8",
doi = "10.1073/pnas.0707197105",
language = "English (US)",
volume = "105",
pages = "347--352",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "1",

}

TY - JOUR

T1 - Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release

AU - Kuner, T.

AU - Li, Y.

AU - Gee, K. R.

AU - Bonewald, L. F.

AU - Augustine, G. J.

PY - 2008/1/8

Y1 - 2008/1/8

N2 - The time at which the N-ethylmaleimide-sensitive factor (NSF) acts during synaptic vesicle (SV) trafficking was identified by time-controlled perturbation of NSF function with a photoactivatable inhibitory peptide. Photolysis of this caged peptide in the squid giant presynaptic terminal caused an abrupt (0.2 s) slowing of the kinetics of the postsynaptic current (PSC) and a more gradual (2-3 s) reduction in PSC amplitude. Based on the rapid rate of these inhibitory effects relative to the speed of SV recycling, we conclude that NSF functions in reactions that immediately precede neurotransmitter release. Our results indicate the locus of SNARE protein recycling in presynaptic terminals and reveal NSF as a potential target for rapid regulation of transmitter release.

AB - The time at which the N-ethylmaleimide-sensitive factor (NSF) acts during synaptic vesicle (SV) trafficking was identified by time-controlled perturbation of NSF function with a photoactivatable inhibitory peptide. Photolysis of this caged peptide in the squid giant presynaptic terminal caused an abrupt (0.2 s) slowing of the kinetics of the postsynaptic current (PSC) and a more gradual (2-3 s) reduction in PSC amplitude. Based on the rapid rate of these inhibitory effects relative to the speed of SV recycling, we conclude that NSF functions in reactions that immediately precede neurotransmitter release. Our results indicate the locus of SNARE protein recycling in presynaptic terminals and reveal NSF as a potential target for rapid regulation of transmitter release.

KW - Caged probes

KW - Exocytosis

KW - Synaptic transmission

KW - Synaptic vesicle cycle

UR - http://www.scopus.com/inward/record.url?scp=38349098186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349098186&partnerID=8YFLogxK

U2 - 10.1073/pnas.0707197105

DO - 10.1073/pnas.0707197105

M3 - Article

C2 - 18172208

AN - SCOPUS:38349098186

VL - 105

SP - 347

EP - 352

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 1

ER -