Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers

María F. Hamilton, Andrew D. Otte, Richard Gregory, Rodolfo Pinal, Andrea Ferreira-Zandoná, Marco C. Bottino

Research output: Contribution to journalArticle

12 Scopus citations


This study aimed to develop and evaluate resin-based experimental dental sealants containing electrospun nylon-6 (N6) and chitosan (CH) fibers in an attempt to improve the physicomechanical properties and provide an antibacterial protective effect, respectively. Electrospun N6 and CH mats were immersed into a resin mixture, light-cured, and then cryomilled to obtain micron-sized resin-modified fiber particles. Different levels of the novel cryomilled particles (i.e. 1, 2.5, and 5% relative to the resin mixture, % by weight) were used to prepare the N6- and CH-containing sealants. A commercial sealant and the experimental resin mixture (unfilled) were used as controls. Flexural strength (FS), Vickers microhardness (VH), and agar diffusion tests were performed. The data were analyzed at the 5% significance level. No significant difference in fiber diameter of N6 (503 ± 31 nm) and CH (595 ± 38 nm) was observed. Upon cryomilling, the resin-modified CH and N6 mats led to the formation of irregularly-shaped particles, with an average diameter of 14.24 μm and 15.87 μm, respectively. CH-5% had significantly higher FS (115.3 ± 1.3 MPa) than all the other groups. CH-1% had significantly higher hardness values (38.3 ± 0.3 VHN) than all the other groups. Collectively, the results indicated that CH-containing sealants presented the highest FS and hardness; however, none of the CH-containing sealants displayed antimicrobial properties.

Original languageEnglish (US)
Pages (from-to)1560-1568
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number8
StatePublished - Nov 1 2015


  • antibacterial
  • chitosan
  • dental sealants
  • electrospinning
  • nylon-6
  • resin

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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