Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers

María F. Hamilton, Andrew D. Otte, Richard Gregory, Rodolfo Pinal, Andrea Ferreira-Zandoná, Marco C. Bottino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This study aimed to develop and evaluate resin-based experimental dental sealants containing electrospun nylon-6 (N6) and chitosan (CH) fibers in an attempt to improve the physicomechanical properties and provide an antibacterial protective effect, respectively. Electrospun N6 and CH mats were immersed into a resin mixture, light-cured, and then cryomilled to obtain micron-sized resin-modified fiber particles. Different levels of the novel cryomilled particles (i.e. 1, 2.5, and 5% relative to the resin mixture, % by weight) were used to prepare the N6- and CH-containing sealants. A commercial sealant and the experimental resin mixture (unfilled) were used as controls. Flexural strength (FS), Vickers microhardness (VH), and agar diffusion tests were performed. The data were analyzed at the 5% significance level. No significant difference in fiber diameter of N6 (503 ± 31 nm) and CH (595 ± 38 nm) was observed. Upon cryomilling, the resin-modified CH and N6 mats led to the formation of irregularly-shaped particles, with an average diameter of 14.24 μm and 15.87 μm, respectively. CH-5% had significantly higher FS (115.3 ± 1.3 MPa) than all the other groups. CH-1% had significantly higher hardness values (38.3 ± 0.3 VHN) than all the other groups. Collectively, the results indicated that CH-containing sealants presented the highest FS and hardness; however, none of the CH-containing sealants displayed antimicrobial properties.

Original languageEnglish (US)
Pages (from-to)1560-1568
Number of pages9
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume103
Issue number8
DOIs
StatePublished - Nov 1 2015

Fingerprint

Pit and Fissure Sealants
Sealants
Chitosan
Nanofibers
Resins
Bending strength
Fibers
Hardness
nylon 6
Microhardness
Agar

Keywords

  • antibacterial
  • chitosan
  • dental sealants
  • electrospinning
  • nylon-6
  • resin

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers. / Hamilton, María F.; Otte, Andrew D.; Gregory, Richard; Pinal, Rodolfo; Ferreira-Zandoná, Andrea; Bottino, Marco C.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 103, No. 8, 01.11.2015, p. 1560-1568.

Research output: Contribution to journalArticle

Hamilton, María F. ; Otte, Andrew D. ; Gregory, Richard ; Pinal, Rodolfo ; Ferreira-Zandoná, Andrea ; Bottino, Marco C. / Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials. 2015 ; Vol. 103, No. 8. pp. 1560-1568.
@article{fe02d16a6faf448aa38d8b5e6fb2c3b7,
title = "Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers",
abstract = "This study aimed to develop and evaluate resin-based experimental dental sealants containing electrospun nylon-6 (N6) and chitosan (CH) fibers in an attempt to improve the physicomechanical properties and provide an antibacterial protective effect, respectively. Electrospun N6 and CH mats were immersed into a resin mixture, light-cured, and then cryomilled to obtain micron-sized resin-modified fiber particles. Different levels of the novel cryomilled particles (i.e. 1, 2.5, and 5{\%} relative to the resin mixture, {\%} by weight) were used to prepare the N6- and CH-containing sealants. A commercial sealant and the experimental resin mixture (unfilled) were used as controls. Flexural strength (FS), Vickers microhardness (VH), and agar diffusion tests were performed. The data were analyzed at the 5{\%} significance level. No significant difference in fiber diameter of N6 (503 ± 31 nm) and CH (595 ± 38 nm) was observed. Upon cryomilling, the resin-modified CH and N6 mats led to the formation of irregularly-shaped particles, with an average diameter of 14.24 μm and 15.87 μm, respectively. CH-5{\%} had significantly higher FS (115.3 ± 1.3 MPa) than all the other groups. CH-1{\%} had significantly higher hardness values (38.3 ± 0.3 VHN) than all the other groups. Collectively, the results indicated that CH-containing sealants presented the highest FS and hardness; however, none of the CH-containing sealants displayed antimicrobial properties.",
keywords = "antibacterial, chitosan, dental sealants, electrospinning, nylon-6, resin",
author = "Hamilton, {Mar{\'i}a F.} and Otte, {Andrew D.} and Richard Gregory and Rodolfo Pinal and Andrea Ferreira-Zandon{\'a} and Bottino, {Marco C.}",
year = "2015",
month = "11",
day = "1",
doi = "10.1002/jbm.b.33342",
language = "English (US)",
volume = "103",
pages = "1560--1568",
journal = "Journal of Biomedical Materials Research",
issn = "1552-4973",
publisher = "Heterocorporation",
number = "8",

}

TY - JOUR

T1 - Physicomechanical and antibacterial properties of experimental resin-based dental sealants modified with nylon-6 and chitosan nanofibers

AU - Hamilton, María F.

AU - Otte, Andrew D.

AU - Gregory, Richard

AU - Pinal, Rodolfo

AU - Ferreira-Zandoná, Andrea

AU - Bottino, Marco C.

PY - 2015/11/1

Y1 - 2015/11/1

N2 - This study aimed to develop and evaluate resin-based experimental dental sealants containing electrospun nylon-6 (N6) and chitosan (CH) fibers in an attempt to improve the physicomechanical properties and provide an antibacterial protective effect, respectively. Electrospun N6 and CH mats were immersed into a resin mixture, light-cured, and then cryomilled to obtain micron-sized resin-modified fiber particles. Different levels of the novel cryomilled particles (i.e. 1, 2.5, and 5% relative to the resin mixture, % by weight) were used to prepare the N6- and CH-containing sealants. A commercial sealant and the experimental resin mixture (unfilled) were used as controls. Flexural strength (FS), Vickers microhardness (VH), and agar diffusion tests were performed. The data were analyzed at the 5% significance level. No significant difference in fiber diameter of N6 (503 ± 31 nm) and CH (595 ± 38 nm) was observed. Upon cryomilling, the resin-modified CH and N6 mats led to the formation of irregularly-shaped particles, with an average diameter of 14.24 μm and 15.87 μm, respectively. CH-5% had significantly higher FS (115.3 ± 1.3 MPa) than all the other groups. CH-1% had significantly higher hardness values (38.3 ± 0.3 VHN) than all the other groups. Collectively, the results indicated that CH-containing sealants presented the highest FS and hardness; however, none of the CH-containing sealants displayed antimicrobial properties.

AB - This study aimed to develop and evaluate resin-based experimental dental sealants containing electrospun nylon-6 (N6) and chitosan (CH) fibers in an attempt to improve the physicomechanical properties and provide an antibacterial protective effect, respectively. Electrospun N6 and CH mats were immersed into a resin mixture, light-cured, and then cryomilled to obtain micron-sized resin-modified fiber particles. Different levels of the novel cryomilled particles (i.e. 1, 2.5, and 5% relative to the resin mixture, % by weight) were used to prepare the N6- and CH-containing sealants. A commercial sealant and the experimental resin mixture (unfilled) were used as controls. Flexural strength (FS), Vickers microhardness (VH), and agar diffusion tests were performed. The data were analyzed at the 5% significance level. No significant difference in fiber diameter of N6 (503 ± 31 nm) and CH (595 ± 38 nm) was observed. Upon cryomilling, the resin-modified CH and N6 mats led to the formation of irregularly-shaped particles, with an average diameter of 14.24 μm and 15.87 μm, respectively. CH-5% had significantly higher FS (115.3 ± 1.3 MPa) than all the other groups. CH-1% had significantly higher hardness values (38.3 ± 0.3 VHN) than all the other groups. Collectively, the results indicated that CH-containing sealants presented the highest FS and hardness; however, none of the CH-containing sealants displayed antimicrobial properties.

KW - antibacterial

KW - chitosan

KW - dental sealants

KW - electrospinning

KW - nylon-6

KW - resin

UR - http://www.scopus.com/inward/record.url?scp=84945448199&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945448199&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.33342

DO - 10.1002/jbm.b.33342

M3 - Article

C2 - 25532852

AN - SCOPUS:84945448199

VL - 103

SP - 1560

EP - 1568

JO - Journal of Biomedical Materials Research

JF - Journal of Biomedical Materials Research

SN - 1552-4973

IS - 8

ER -