Physiological stretch induced proliferation of human urothelial cells via integrin α6-FAK signaling pathway

Xiaoshuai Gao, Tangqiang Wei, Banghua Liao, Jianzhong Ai, Liang Zhou, Lina Gong, Yuntian Chen, Qing He, Liang Cheng, Kunjie Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aims: To test a kind of stretch pattern which is the optimum stress parameter to promote human urothelial cells (HUCs) proliferation, and to investigate the roles of integrin subunits and their pathway in the HUCs proliferation induced by physiological stretch. Methods: HUCs were seeded on silicone membrane, and subjected to four kinds of stretch (0,5%,10%,15% elongation) for 24 h, as controlled by a BioDynamic® bioreactor. Cell proliferation, viability and cycle distribution were examined using Cell Counting Kit-8 and flow cytometry, respectively. The gene and protein expression of integrin subunits and focal adhesion kinase (FAK) in each group were assessed by Real-time PCR(RT-PCR) and western blot, respectively. Small interfering RNAs (siRNA) were applied to knockdown integrin α6 and FAK expression in HUCs, and FAK inhibitor was used to validate the role of α6 and FAK in cell proliferation under physiological stretch. Results: The proliferation of HUCs were highest in the 5% elongation group compared to static control, 10% and 15% elongation group. RT-PCR and western blot showed that 5% cyclic stretch significantly promoted the expression of integrin α6 and FAK. The stretch-induced cell proliferation and FAK expression was inhibited by siRNA of integrin α6. Further study with FAK inhibitor revealed that elongation promoted proliferation though integrin α6 and FAK signaling pathway. Conclusions: Physiological stretch induced HUCs proliferation via integrin α6-FAK signaling pathway, and 5% elongation may be the optimal stress parameter to promote the cell proliferation.

Original languageEnglish (US)
Pages (from-to)2114-2120
Number of pages7
JournalNeurourology and Urodynamics
Volume37
Issue number7
DOIs
StatePublished - Sep 1 2018
Externally publishedYes

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Integrins
Cell Proliferation
Small Interfering RNA
Real-Time Polymerase Chain Reaction
Western Blotting
Bioreactors
Silicones
Cell Adhesion
Cell Survival
Cell Cycle
Flow Cytometry
Gene Expression
Membranes

Keywords

  • bladder outlet obstruction
  • cell proliferation
  • focal adhesion kinase
  • human urothelial cells
  • integrin
  • simulated physiological stretch

ASJC Scopus subject areas

  • Clinical Neurology
  • Urology

Cite this

Physiological stretch induced proliferation of human urothelial cells via integrin α6-FAK signaling pathway. / Gao, Xiaoshuai; Wei, Tangqiang; Liao, Banghua; Ai, Jianzhong; Zhou, Liang; Gong, Lina; Chen, Yuntian; He, Qing; Cheng, Liang; Wang, Kunjie.

In: Neurourology and Urodynamics, Vol. 37, No. 7, 01.09.2018, p. 2114-2120.

Research output: Contribution to journalArticle

Gao, Xiaoshuai ; Wei, Tangqiang ; Liao, Banghua ; Ai, Jianzhong ; Zhou, Liang ; Gong, Lina ; Chen, Yuntian ; He, Qing ; Cheng, Liang ; Wang, Kunjie. / Physiological stretch induced proliferation of human urothelial cells via integrin α6-FAK signaling pathway. In: Neurourology and Urodynamics. 2018 ; Vol. 37, No. 7. pp. 2114-2120.
@article{28a7119ff7b5472faa46d0ca67d0bd15,
title = "Physiological stretch induced proliferation of human urothelial cells via integrin α6-FAK signaling pathway",
abstract = "Aims: To test a kind of stretch pattern which is the optimum stress parameter to promote human urothelial cells (HUCs) proliferation, and to investigate the roles of integrin subunits and their pathway in the HUCs proliferation induced by physiological stretch. Methods: HUCs were seeded on silicone membrane, and subjected to four kinds of stretch (0,5{\%},10{\%},15{\%} elongation) for 24 h, as controlled by a BioDynamic{\circledR} bioreactor. Cell proliferation, viability and cycle distribution were examined using Cell Counting Kit-8 and flow cytometry, respectively. The gene and protein expression of integrin subunits and focal adhesion kinase (FAK) in each group were assessed by Real-time PCR(RT-PCR) and western blot, respectively. Small interfering RNAs (siRNA) were applied to knockdown integrin α6 and FAK expression in HUCs, and FAK inhibitor was used to validate the role of α6 and FAK in cell proliferation under physiological stretch. Results: The proliferation of HUCs were highest in the 5{\%} elongation group compared to static control, 10{\%} and 15{\%} elongation group. RT-PCR and western blot showed that 5{\%} cyclic stretch significantly promoted the expression of integrin α6 and FAK. The stretch-induced cell proliferation and FAK expression was inhibited by siRNA of integrin α6. Further study with FAK inhibitor revealed that elongation promoted proliferation though integrin α6 and FAK signaling pathway. Conclusions: Physiological stretch induced HUCs proliferation via integrin α6-FAK signaling pathway, and 5{\%} elongation may be the optimal stress parameter to promote the cell proliferation.",
keywords = "bladder outlet obstruction, cell proliferation, focal adhesion kinase, human urothelial cells, integrin, simulated physiological stretch",
author = "Xiaoshuai Gao and Tangqiang Wei and Banghua Liao and Jianzhong Ai and Liang Zhou and Lina Gong and Yuntian Chen and Qing He and Liang Cheng and Kunjie Wang",
year = "2018",
month = "9",
day = "1",
doi = "10.1002/nau.23572",
language = "English (US)",
volume = "37",
pages = "2114--2120",
journal = "Neurourology and Urodynamics",
issn = "0733-2467",
publisher = "Wiley-Liss Inc.",
number = "7",

}

TY - JOUR

T1 - Physiological stretch induced proliferation of human urothelial cells via integrin α6-FAK signaling pathway

AU - Gao, Xiaoshuai

AU - Wei, Tangqiang

AU - Liao, Banghua

AU - Ai, Jianzhong

AU - Zhou, Liang

AU - Gong, Lina

AU - Chen, Yuntian

AU - He, Qing

AU - Cheng, Liang

AU - Wang, Kunjie

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Aims: To test a kind of stretch pattern which is the optimum stress parameter to promote human urothelial cells (HUCs) proliferation, and to investigate the roles of integrin subunits and their pathway in the HUCs proliferation induced by physiological stretch. Methods: HUCs were seeded on silicone membrane, and subjected to four kinds of stretch (0,5%,10%,15% elongation) for 24 h, as controlled by a BioDynamic® bioreactor. Cell proliferation, viability and cycle distribution were examined using Cell Counting Kit-8 and flow cytometry, respectively. The gene and protein expression of integrin subunits and focal adhesion kinase (FAK) in each group were assessed by Real-time PCR(RT-PCR) and western blot, respectively. Small interfering RNAs (siRNA) were applied to knockdown integrin α6 and FAK expression in HUCs, and FAK inhibitor was used to validate the role of α6 and FAK in cell proliferation under physiological stretch. Results: The proliferation of HUCs were highest in the 5% elongation group compared to static control, 10% and 15% elongation group. RT-PCR and western blot showed that 5% cyclic stretch significantly promoted the expression of integrin α6 and FAK. The stretch-induced cell proliferation and FAK expression was inhibited by siRNA of integrin α6. Further study with FAK inhibitor revealed that elongation promoted proliferation though integrin α6 and FAK signaling pathway. Conclusions: Physiological stretch induced HUCs proliferation via integrin α6-FAK signaling pathway, and 5% elongation may be the optimal stress parameter to promote the cell proliferation.

AB - Aims: To test a kind of stretch pattern which is the optimum stress parameter to promote human urothelial cells (HUCs) proliferation, and to investigate the roles of integrin subunits and their pathway in the HUCs proliferation induced by physiological stretch. Methods: HUCs were seeded on silicone membrane, and subjected to four kinds of stretch (0,5%,10%,15% elongation) for 24 h, as controlled by a BioDynamic® bioreactor. Cell proliferation, viability and cycle distribution were examined using Cell Counting Kit-8 and flow cytometry, respectively. The gene and protein expression of integrin subunits and focal adhesion kinase (FAK) in each group were assessed by Real-time PCR(RT-PCR) and western blot, respectively. Small interfering RNAs (siRNA) were applied to knockdown integrin α6 and FAK expression in HUCs, and FAK inhibitor was used to validate the role of α6 and FAK in cell proliferation under physiological stretch. Results: The proliferation of HUCs were highest in the 5% elongation group compared to static control, 10% and 15% elongation group. RT-PCR and western blot showed that 5% cyclic stretch significantly promoted the expression of integrin α6 and FAK. The stretch-induced cell proliferation and FAK expression was inhibited by siRNA of integrin α6. Further study with FAK inhibitor revealed that elongation promoted proliferation though integrin α6 and FAK signaling pathway. Conclusions: Physiological stretch induced HUCs proliferation via integrin α6-FAK signaling pathway, and 5% elongation may be the optimal stress parameter to promote the cell proliferation.

KW - bladder outlet obstruction

KW - cell proliferation

KW - focal adhesion kinase

KW - human urothelial cells

KW - integrin

KW - simulated physiological stretch

UR - http://www.scopus.com/inward/record.url?scp=85053479316&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85053479316&partnerID=8YFLogxK

U2 - 10.1002/nau.23572

DO - 10.1002/nau.23572

M3 - Article

C2 - 29953644

AN - SCOPUS:85053479316

VL - 37

SP - 2114

EP - 2120

JO - Neurourology and Urodynamics

JF - Neurourology and Urodynamics

SN - 0733-2467

IS - 7

ER -