Pigment epithelium-derived factor in idiopathic pulmonary fibrosis: A role in aberrant angiogenesis

Gregory P. Cosgrove, Kevin K. Brown, William P. Schiemann, Amanda E. Serls, Jane E. Parr, Mark W. Geraci, Marvin I. Schwarz, Carlyne D. Cool, G. Scott Worthen

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

Pigment epithelium-derived factor (PEDF) is a 50-kD protein with angiostatic and neurotrophic activities that regulates vascular development within the eye. PEDF expression was increased in the lungs of patients with idiopathic pulmonary fibrosis (IPF) based on microarray analyses. Angiogenesis has been implicated in the pathogenesis of fibrotic lung diseases, we therefore hypothesized that regional abnormalities in vascularization occur in IPF as a result of an imbalance between PEDF and vascular endothelial growth factor. We demonstrated that vascular density is regionally decreased in IPF within the fibroblastic foci, and that within these areas PEDF was increased, whereas vascular endothelial growth factor was decreased. PEDF colocalized with the fibrogenic cytokine, transforming growth factor (TGF)-β1, particularly within the fibrotic interstitium and the fibroblastic focus, and prominently within the epithelium directly overlying the fibroblastic focus. This suggested that TGF-β1 might regulate PEDF expression. Using 3T3-L1 fibroblasts and human lung fibroblasts, we showed that PEDF was indeed a TGF-β1 target gene. Collectively, our findings implicate PEDF as a regulator of pulmonary angiogenesis and an important mediator in IPF.

Original languageEnglish (US)
Pages (from-to)242-251
Number of pages10
JournalAmerican journal of respiratory and critical care medicine
Volume170
Issue number3
StatePublished - Aug 1 2004

Fingerprint

Idiopathic Pulmonary Fibrosis
Transforming Growth Factors
Angiostatic Proteins
Lung
Vascular Endothelial Growth Factor A
Blood Vessels
Fibroblasts
pigment epithelium-derived factor
Nerve Growth Factors
Microarray Analysis
Lung Diseases
Epithelium
Cytokines

Keywords

  • Cryptogenic organizing pneumonia
  • Pigment epithelium-derived factor
  • Transforming growth factor-β1
  • Vascular endothelial growth factor

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Cosgrove, G. P., Brown, K. K., Schiemann, W. P., Serls, A. E., Parr, J. E., Geraci, M. W., ... Worthen, G. S. (2004). Pigment epithelium-derived factor in idiopathic pulmonary fibrosis: A role in aberrant angiogenesis. American journal of respiratory and critical care medicine, 170(3), 242-251.

Pigment epithelium-derived factor in idiopathic pulmonary fibrosis : A role in aberrant angiogenesis. / Cosgrove, Gregory P.; Brown, Kevin K.; Schiemann, William P.; Serls, Amanda E.; Parr, Jane E.; Geraci, Mark W.; Schwarz, Marvin I.; Cool, Carlyne D.; Worthen, G. Scott.

In: American journal of respiratory and critical care medicine, Vol. 170, No. 3, 01.08.2004, p. 242-251.

Research output: Contribution to journalArticle

Cosgrove, GP, Brown, KK, Schiemann, WP, Serls, AE, Parr, JE, Geraci, MW, Schwarz, MI, Cool, CD & Worthen, GS 2004, 'Pigment epithelium-derived factor in idiopathic pulmonary fibrosis: A role in aberrant angiogenesis', American journal of respiratory and critical care medicine, vol. 170, no. 3, pp. 242-251.
Cosgrove, Gregory P. ; Brown, Kevin K. ; Schiemann, William P. ; Serls, Amanda E. ; Parr, Jane E. ; Geraci, Mark W. ; Schwarz, Marvin I. ; Cool, Carlyne D. ; Worthen, G. Scott. / Pigment epithelium-derived factor in idiopathic pulmonary fibrosis : A role in aberrant angiogenesis. In: American journal of respiratory and critical care medicine. 2004 ; Vol. 170, No. 3. pp. 242-251.
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