Pilot clinical study to assess caries lesion activity using quantitative light-induced fluorescence during dehydration

Masatoshi Ando, Andrea G. Ferreira-Zandoná, George J. Eckert, Domenick Zero, George K. Stookey

Research output: Contribution to journalArticle

9 Scopus citations


This study aimed to evaluate the ability of quantitative light-induced fluorescence (QLF) to assess caries lesion activity using visual examination (VE) as the gold standard. Twenty-four visible white spot lesions on buccal surfaces were examined from 23 children, ages 9 to 14 years. At baseline, the surface was hydrated with water, and thereafter, it was dehydrated with continuous compressed air during image acquisition. QLF images were acquired at 0 (baseline), 5, and 15 s. QLF variables [QLFV: Fluorescence loss (ΔF), lesion size (S), ΔQ: ΔF × S] was recorded. Changes-in-QLFV per second (ΔQLFV) were determined: ΔQLFV = (QLFVN - QLFVBaseline)/N, where N indicates dehydration time. One experienced dentist conducted VE independently using a dental unit's light, compressed air, and explorer. QLFV and ΔQLFV of the active group (n = 11) were compared with those of the inactive group (n = 13) using two-sample t -tests. As the surface was dehydrated, S and ΔQ values of the active group increased, whereas QLFV of the inactive group showed only a small change. ΔQLFV of the active group were larger than those of the inactive group; however, the difference did not reach statistical significance (p > 0.11). Within the limitations of this study, QLF data indicated increments for lesions designated as active and minimal change for lesions defined as inactive.

Original languageEnglish (US)
Article number035005
JournalJournal of Biomedical Optics
Issue number3
StatePublished - Mar 1 2017


ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Biomedical Engineering

Cite this