Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions

Jeongheon Yoon, Hongsheng Wang, Yong Chan Kim, Momoko Yoshimoto, Sadia Abbasi, Herbert C. Morse

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Plasma cell alloantigen 1 (PC1), also known as ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), is an enzyme involved primarily in hydrolysis of adenosine triphosphate at the cell surface. Although the expression pattern of PC1 is relatively broad, its expression in B cells is found at significant levels only in terminally differentiated germinal center B cells, plasma cells and a subset of B-1a cells in mice. Here we describe studies designed to determine whether expression of PC1 might define novel populations of human B cells with similarities to mouse B cells. We found that PC1 is expressed in small populations of human B lineage cells in peripheral blood, cord blood, tonsils, bone marrow and pediatric peritoneal fluid, with the highest levels in plasma cells. The characteristics of human PC1+ B cells differ from mouse peritoneal B-1a subsets and from features of the human CD20+CD27+CD43+CD70- B-cell subset proposed to be human B-1 cells. Expression of PC1 was greatly increased in B cells stimulated with the combination of CD40 ligand, interleukin (IL)-4 and IL-21. In addition, PC1+ B cells activated CD4+ T regulatory cells. ENPP1 thus defines a subset of human B cells that differs significantly from mouse peritoneal B-1a and proposed human B-1 cells.Immunology and Cell Biology advance online publication, 31 May 2016; doi:10.1038/icb.2016.31.

Original languageEnglish (US)
JournalImmunology and Cell Biology
DOIs
StateAccepted/In press - May 31 2016
Externally publishedYes

Fingerprint

B-Lymphocyte Subsets
Isoantigens
Plasma Cells
B-Lymphocytes
ectonucleotide pyrophosphatase phosphodiesterase 1
CD40 Ligand
Germinal Center
Ascitic Fluid
Palatine Tonsil
Regulatory T-Lymphocytes
Allergy and Immunology
Fetal Blood
Interleukin-4
Population
Cell Biology
Publications
Hydrolysis
Adenosine Triphosphate
Bone Marrow
Pediatrics

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)
  • Cell Biology

Cite this

Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions. / Yoon, Jeongheon; Wang, Hongsheng; Kim, Yong Chan; Yoshimoto, Momoko; Abbasi, Sadia; Morse, Herbert C.

In: Immunology and Cell Biology, 31.05.2016.

Research output: Contribution to journalArticle

Yoon, Jeongheon ; Wang, Hongsheng ; Kim, Yong Chan ; Yoshimoto, Momoko ; Abbasi, Sadia ; Morse, Herbert C. / Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions. In: Immunology and Cell Biology. 2016.
@article{8935a3241839435c962f25b6f57bc308,
title = "Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions",
abstract = "Plasma cell alloantigen 1 (PC1), also known as ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), is an enzyme involved primarily in hydrolysis of adenosine triphosphate at the cell surface. Although the expression pattern of PC1 is relatively broad, its expression in B cells is found at significant levels only in terminally differentiated germinal center B cells, plasma cells and a subset of B-1a cells in mice. Here we describe studies designed to determine whether expression of PC1 might define novel populations of human B cells with similarities to mouse B cells. We found that PC1 is expressed in small populations of human B lineage cells in peripheral blood, cord blood, tonsils, bone marrow and pediatric peritoneal fluid, with the highest levels in plasma cells. The characteristics of human PC1+ B cells differ from mouse peritoneal B-1a subsets and from features of the human CD20+CD27+CD43+CD70- B-cell subset proposed to be human B-1 cells. Expression of PC1 was greatly increased in B cells stimulated with the combination of CD40 ligand, interleukin (IL)-4 and IL-21. In addition, PC1+ B cells activated CD4+ T regulatory cells. ENPP1 thus defines a subset of human B cells that differs significantly from mouse peritoneal B-1a and proposed human B-1 cells.Immunology and Cell Biology advance online publication, 31 May 2016; doi:10.1038/icb.2016.31.",
author = "Jeongheon Yoon and Hongsheng Wang and Kim, {Yong Chan} and Momoko Yoshimoto and Sadia Abbasi and Morse, {Herbert C.}",
year = "2016",
month = "5",
day = "31",
doi = "10.1038/icb.2016.31",
language = "English (US)",
journal = "Immunology and Cell Biology",
issn = "0818-9641",
publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Plasma cell alloantigen ENPP1 is expressed by a subset of human B cells with potential regulatory functions

AU - Yoon, Jeongheon

AU - Wang, Hongsheng

AU - Kim, Yong Chan

AU - Yoshimoto, Momoko

AU - Abbasi, Sadia

AU - Morse, Herbert C.

PY - 2016/5/31

Y1 - 2016/5/31

N2 - Plasma cell alloantigen 1 (PC1), also known as ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), is an enzyme involved primarily in hydrolysis of adenosine triphosphate at the cell surface. Although the expression pattern of PC1 is relatively broad, its expression in B cells is found at significant levels only in terminally differentiated germinal center B cells, plasma cells and a subset of B-1a cells in mice. Here we describe studies designed to determine whether expression of PC1 might define novel populations of human B cells with similarities to mouse B cells. We found that PC1 is expressed in small populations of human B lineage cells in peripheral blood, cord blood, tonsils, bone marrow and pediatric peritoneal fluid, with the highest levels in plasma cells. The characteristics of human PC1+ B cells differ from mouse peritoneal B-1a subsets and from features of the human CD20+CD27+CD43+CD70- B-cell subset proposed to be human B-1 cells. Expression of PC1 was greatly increased in B cells stimulated with the combination of CD40 ligand, interleukin (IL)-4 and IL-21. In addition, PC1+ B cells activated CD4+ T regulatory cells. ENPP1 thus defines a subset of human B cells that differs significantly from mouse peritoneal B-1a and proposed human B-1 cells.Immunology and Cell Biology advance online publication, 31 May 2016; doi:10.1038/icb.2016.31.

AB - Plasma cell alloantigen 1 (PC1), also known as ENPP1 (ectonucleotide pyrophosphatase/phosphodiesterase 1), is an enzyme involved primarily in hydrolysis of adenosine triphosphate at the cell surface. Although the expression pattern of PC1 is relatively broad, its expression in B cells is found at significant levels only in terminally differentiated germinal center B cells, plasma cells and a subset of B-1a cells in mice. Here we describe studies designed to determine whether expression of PC1 might define novel populations of human B cells with similarities to mouse B cells. We found that PC1 is expressed in small populations of human B lineage cells in peripheral blood, cord blood, tonsils, bone marrow and pediatric peritoneal fluid, with the highest levels in plasma cells. The characteristics of human PC1+ B cells differ from mouse peritoneal B-1a subsets and from features of the human CD20+CD27+CD43+CD70- B-cell subset proposed to be human B-1 cells. Expression of PC1 was greatly increased in B cells stimulated with the combination of CD40 ligand, interleukin (IL)-4 and IL-21. In addition, PC1+ B cells activated CD4+ T regulatory cells. ENPP1 thus defines a subset of human B cells that differs significantly from mouse peritoneal B-1a and proposed human B-1 cells.Immunology and Cell Biology advance online publication, 31 May 2016; doi:10.1038/icb.2016.31.

UR - http://www.scopus.com/inward/record.url?scp=84973131053&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973131053&partnerID=8YFLogxK

U2 - 10.1038/icb.2016.31

DO - 10.1038/icb.2016.31

M3 - Article

JO - Immunology and Cell Biology

JF - Immunology and Cell Biology

SN - 0818-9641

ER -