Plasma CXCL9 elevations correlate with chronic GVHD diagnosis

Carrie L. Kitko, John E. Levine, Barry E. Storer, Xiaoyu Chai, David A. Fox, Thomas M. Braun, Daniel R. Couriel, Paul J. Martin, Mary E. Flowers, John A. Hansen, Lawrence Chang, Megan Conlon, Bryan J. Fiema, Rachel Morgan, Prae Pongtornpipat, Kelly Lamiman, James L.M. Ferrara, Stephanie J. Lee, Sophie Paczesny

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

There are no validated biomarkers for chronic GVHD (cGVHD). We used a protein microarray and subsequent sequential enzyme-linked immunosorbent assay to compare 17 patients with treatment-refractory de novo-onset cGVHD and 18 time-matched control patients without acute or chronicGVHDto identify 5 candidate proteins that distinguished cGVHD from no cGVHD: CXCL9, IL2Ra, elafin, CD13, and BAFF. We then assessed the discriminatory value of each protein individually and in composite panels in a validation cohort (n5109). CXCL9wasfoundtohavethehighest discriminatory valuewithanareaunder the receiver operating characteristic curve of 0.83 (95% confidence interval, 0.74-0.91).CXCL9 plasma concentrations above the median were associatedwith a higher frequency of cGVHD even after adjustment for other factors related todevelopingcGVHDincludingage, diagnosis, donor source, and degree of HLA matching (71% vs 20%; P > .001). A separate validation cohort from a different transplant center (n 5 211) confirmed that CXCL9 plasma concentrations abovethemedianwereassociatedwithmorefrequentnewlydiagnosedcGVHDafteradjustingfo r theaforementionedfactors (84% vs 60%; P 5 .001). Our results confirmthat CXCL9 is elevated in patients with newly diagnosed cGVHD.

Original languageEnglish (US)
Pages (from-to)786-793
Number of pages8
JournalBlood
Volume123
Issue number5
DOIs
StatePublished - Jan 30 2014

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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