Plasma proteome profiling of a mouse model of breast cancer identifies a set of up-regulated proteins in common with human breast cancer Cells

Sharon J. Pitteri, Vitor M. Faca, Karen S. Kelly-Spratt, A. Erik Kasarda, Hong Wang, Qing Zhang, Lisa Newcomb, Alexei Krasnoselsky, Sophie Paczesny, Gina Choi, Matthew Fitzgibbon, Martin W. Mcintosh, Christopher J. Kemp, Samir M. Hanash

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We have applied an in-depth quantitative proteomic approach, combining isotopic labeling extensive intact protein separation and mass spectrometry, for high confidence identification of protein changes in plasmas from a mouse model of breast cancer. We hypothesized that a wide spectrum of proteins may be up-regulated in plasma with tumor development and that comparisons with proteins expressed in human breast cancer cell lines may identify a subset of up-regulated proteins in common with proteins expressed in breast cancer cell lines that may represent candidate biomarkers for breast cancer. Plasma from PyMT transgenic tumor-bearing mice and matched controls were obtained at two time points during tumor growth. A total of 133 proteins were found to be increased by 1.5-fold or greater at one or both time points. A comparison of this set of proteins with published findings from proteomic analysis of human breast cancer cell lines yielded 49 proteins with increased levels in mouse plasma that were identified in breast cancer cell lines. Pathway analysis comparing the subset of up-regulated proteins known to be expressed in breast cancer cell lines with other up-regulated proteins indicated a cancer related function for the former and a host-response function for the latter. We conclude that integration of proteomic findings from mouse models of breast cancer and from human breast cancer cell lines may help identify a subset of proteins released by breast cancer cells into the circulation and that occur at increased levels in breast cancer.

Original languageEnglish (US)
Pages (from-to)1481-1489
Number of pages9
JournalJournal of Proteome Research
Volume7
Issue number4
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Proteome
Cells
Breast Neoplasms
Plasmas
Proteins
Cell Line
Proteomics
Tumors
Neoplasms
Bearings (structural)
Biomarkers
Labeling
Mass spectrometry
Mass Spectrometry

Keywords

  • Breast cancer
  • Fractionation
  • Mass spectrometry
  • Mouse model
  • Proteomics
  • Quantitative analysis

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Genetics
  • Biotechnology

Cite this

Plasma proteome profiling of a mouse model of breast cancer identifies a set of up-regulated proteins in common with human breast cancer Cells. / Pitteri, Sharon J.; Faca, Vitor M.; Kelly-Spratt, Karen S.; Kasarda, A. Erik; Wang, Hong; Zhang, Qing; Newcomb, Lisa; Krasnoselsky, Alexei; Paczesny, Sophie; Choi, Gina; Fitzgibbon, Matthew; Mcintosh, Martin W.; Kemp, Christopher J.; Hanash, Samir M.

In: Journal of Proteome Research, Vol. 7, No. 4, 04.2008, p. 1481-1489.

Research output: Contribution to journalArticle

Pitteri, SJ, Faca, VM, Kelly-Spratt, KS, Kasarda, AE, Wang, H, Zhang, Q, Newcomb, L, Krasnoselsky, A, Paczesny, S, Choi, G, Fitzgibbon, M, Mcintosh, MW, Kemp, CJ & Hanash, SM 2008, 'Plasma proteome profiling of a mouse model of breast cancer identifies a set of up-regulated proteins in common with human breast cancer Cells', Journal of Proteome Research, vol. 7, no. 4, pp. 1481-1489. https://doi.org/10.1021/pr7007994
Pitteri, Sharon J. ; Faca, Vitor M. ; Kelly-Spratt, Karen S. ; Kasarda, A. Erik ; Wang, Hong ; Zhang, Qing ; Newcomb, Lisa ; Krasnoselsky, Alexei ; Paczesny, Sophie ; Choi, Gina ; Fitzgibbon, Matthew ; Mcintosh, Martin W. ; Kemp, Christopher J. ; Hanash, Samir M. / Plasma proteome profiling of a mouse model of breast cancer identifies a set of up-regulated proteins in common with human breast cancer Cells. In: Journal of Proteome Research. 2008 ; Vol. 7, No. 4. pp. 1481-1489.
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