Abstract
The authors examined the effect of insulin-like growth factor I (IGF I), epidermal growth factor (EGF), and acidic fibroblast growth factor (AFGF) on the synthesis by human retinal endothelial cells (HREC) of plasminogen activators (PA; tissue-type [t-PA] and urokinase-type [u-PA]) and plasminogen activator inhibitor (PAI). Immunologic and functional assays for t-PA, u-PA, and PAI were conducted with cell lines derived from three diabetics and three nondiabetic controls. Confluent HREC of nondiabetic origin did not respond to IGF I (100 ng/ml) with any change of t-PA antigen in the medium (10.7 ± 1.1 ng/ml unstimulated versus 10.1 ± 0.8 ng/ml stimulated, P = not significant). Likewise AFGF and EGF caused no significant change of t-PA levels. Both IGF I and EGF caused a significant increase of t-PA from HREC of diabetic origin (9.6 ± 0.8 ng/ml unstimulated versus 16.6 ± 1.9 ng/ml IGF I-stimulated, P <0.001, and 14.6 ± 2.7 ng/ml EGF-stimulated, P <0.005). Supplementation of AFGF had no effect on HREC of diabetic origin. In confluent cultures, only small quantities of u-PA were detected. After wounding confluent cultures, u-PA activity was associated with cells migrating from the wound edges. Functional PA activity was also measured by chromogenic assay. Results further supported a predominance of t-PA activity being produced by confluent HREC in culture. These results suggest that modulation of PA production by HREC is influenced by exposure to growth factors, by the state of confluency, and the origin of the cells (diabetic versus nondiabetic).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 53-64 |
| Number of pages | 12 |
| Journal | Investigative Ophthalmology and Visual Science |
| Volume | 32 |
| Issue number | 1 |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Ophthalmology
Cite this
Plasminogen activator production by human retinal endothelial cells of nondiabetic and diabetic origin. / Grant, M. B.; Guay, C.
In: Investigative Ophthalmology and Visual Science, Vol. 32, No. 1, 1991, p. 53-64.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Plasminogen activator production by human retinal endothelial cells of nondiabetic and diabetic origin
AU - Grant, M. B.
AU - Guay, C.
PY - 1991
Y1 - 1991
N2 - The authors examined the effect of insulin-like growth factor I (IGF I), epidermal growth factor (EGF), and acidic fibroblast growth factor (AFGF) on the synthesis by human retinal endothelial cells (HREC) of plasminogen activators (PA; tissue-type [t-PA] and urokinase-type [u-PA]) and plasminogen activator inhibitor (PAI). Immunologic and functional assays for t-PA, u-PA, and PAI were conducted with cell lines derived from three diabetics and three nondiabetic controls. Confluent HREC of nondiabetic origin did not respond to IGF I (100 ng/ml) with any change of t-PA antigen in the medium (10.7 ± 1.1 ng/ml unstimulated versus 10.1 ± 0.8 ng/ml stimulated, P = not significant). Likewise AFGF and EGF caused no significant change of t-PA levels. Both IGF I and EGF caused a significant increase of t-PA from HREC of diabetic origin (9.6 ± 0.8 ng/ml unstimulated versus 16.6 ± 1.9 ng/ml IGF I-stimulated, P <0.001, and 14.6 ± 2.7 ng/ml EGF-stimulated, P <0.005). Supplementation of AFGF had no effect on HREC of diabetic origin. In confluent cultures, only small quantities of u-PA were detected. After wounding confluent cultures, u-PA activity was associated with cells migrating from the wound edges. Functional PA activity was also measured by chromogenic assay. Results further supported a predominance of t-PA activity being produced by confluent HREC in culture. These results suggest that modulation of PA production by HREC is influenced by exposure to growth factors, by the state of confluency, and the origin of the cells (diabetic versus nondiabetic).
AB - The authors examined the effect of insulin-like growth factor I (IGF I), epidermal growth factor (EGF), and acidic fibroblast growth factor (AFGF) on the synthesis by human retinal endothelial cells (HREC) of plasminogen activators (PA; tissue-type [t-PA] and urokinase-type [u-PA]) and plasminogen activator inhibitor (PAI). Immunologic and functional assays for t-PA, u-PA, and PAI were conducted with cell lines derived from three diabetics and three nondiabetic controls. Confluent HREC of nondiabetic origin did not respond to IGF I (100 ng/ml) with any change of t-PA antigen in the medium (10.7 ± 1.1 ng/ml unstimulated versus 10.1 ± 0.8 ng/ml stimulated, P = not significant). Likewise AFGF and EGF caused no significant change of t-PA levels. Both IGF I and EGF caused a significant increase of t-PA from HREC of diabetic origin (9.6 ± 0.8 ng/ml unstimulated versus 16.6 ± 1.9 ng/ml IGF I-stimulated, P <0.001, and 14.6 ± 2.7 ng/ml EGF-stimulated, P <0.005). Supplementation of AFGF had no effect on HREC of diabetic origin. In confluent cultures, only small quantities of u-PA were detected. After wounding confluent cultures, u-PA activity was associated with cells migrating from the wound edges. Functional PA activity was also measured by chromogenic assay. Results further supported a predominance of t-PA activity being produced by confluent HREC in culture. These results suggest that modulation of PA production by HREC is influenced by exposure to growth factors, by the state of confluency, and the origin of the cells (diabetic versus nondiabetic).
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M3 - Article
VL - 32
SP - 53
EP - 64
JO - Investigative Ophthalmology and Visual Science
JF - Investigative Ophthalmology and Visual Science
SN - 0146-0404
IS - 1
ER -