Plasmoelectronic-Based Ultrasensitive Assay of Tumor Suppressor microRNAs Directly in Patient Plasma

Design of Highly Specific Early Cancer Diagnostic Technology

Thakshila Liyanage, Adrianna N. Masterson, Hector H. Oyem, Hristos Kaimakliotis, Hang Nguyen, Rajesh Sardar

Research output: Contribution to journalArticle

Abstract

It is becoming understood that microRNAs hold great promise for noninvasive liquid biopsies for screening for different types of cancer, but current state-of-the-art RT-PCR and microarray techniques have sensitivity limitations that currently restrict their use. Herein, we report a new transduction mechanism involving delocalization of photoexcited conduction electrons wave function of gold triangular nanoprism (Au TNP) in the presence of -ssDNA/microRNA duplexes. This plasmoelectronic effect increases the electronic dimension of Au TNPs and substantially affects their localized surface plasmon resonance (LSPR) properties that together allow us to achieve a sensitivity for microRNA assay as low as 140 zeptomolar concentrations for our nanoplasmonic sensors. We show that the position of a single base-pair mismatch in the -ssDNA/microRNA duplex dramatically alters the LSPR properties and detection sensitivity. The unprecedentedly high sensitivity of nanoplasmonic sensors has allowed us to assay four different microRNAs (microRNA-10b, -182, -143, and -145) from bladder cancer patient plasma (50 μL/sample). For the first time, we demonstrate the utility of a label-free, nanoplasmonic sensor in quantification of tumor suppressor microRNAs, the level of tumor suppressor microRNAs goes down in a cancer patient as compared to normal healthy individuals, in metastatic and nonmetastatic bladder cancer patient plasma. Our statistical analysis of patient samples unequivocally suggests that the tumor suppressor microRNAs are more specific biomarkers (p-value of <0.0001) than oncogenic microRNAs for differentiation between metastatic and nonmetastatic bladder cancer, and nonmetastatic cancer from healthy individuals. This work demonstrating the electron wave functions delocalization dependent ultrasensitive LSPR properties of noble metal nanoparticles has a great potential for fabrication of miniaturized and extremely powerful sensors to investigate microRNA properties in other cancers (for example breast, lung, and pancreatic) through liquid biopsy.

Original languageEnglish (US)
Pages (from-to)1894-1903
Number of pages10
JournalAnalytical Chemistry
Volume91
Issue number3
DOIs
StatePublished - Feb 5 2019

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MicroRNAs
Tumors
Assays
Plasmas
Surface plasmon resonance
Biopsy
Sensors
Wave functions
Electrons
Metal nanoparticles
Liquids
Biomarkers
Microarrays
Precious metals
Gold
Labels
Statistical methods
Screening
Fabrication

ASJC Scopus subject areas

  • Analytical Chemistry

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Plasmoelectronic-Based Ultrasensitive Assay of Tumor Suppressor microRNAs Directly in Patient Plasma : Design of Highly Specific Early Cancer Diagnostic Technology. / Liyanage, Thakshila; Masterson, Adrianna N.; Oyem, Hector H.; Kaimakliotis, Hristos; Nguyen, Hang; Sardar, Rajesh.

In: Analytical Chemistry, Vol. 91, No. 3, 05.02.2019, p. 1894-1903.

Research output: Contribution to journalArticle

Liyanage, Thakshila ; Masterson, Adrianna N. ; Oyem, Hector H. ; Kaimakliotis, Hristos ; Nguyen, Hang ; Sardar, Rajesh. / Plasmoelectronic-Based Ultrasensitive Assay of Tumor Suppressor microRNAs Directly in Patient Plasma : Design of Highly Specific Early Cancer Diagnostic Technology. In: Analytical Chemistry. 2019 ; Vol. 91, No. 3. pp. 1894-1903.
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