Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain

Sulayman D. Dib-Hajj, Jenny Fjell, Theodore Cummins, Zheng Zheng, Kaj Fried, Robert Lamotte, Joel A. Black, Stephen G. Waxman

Research output: Contribution to journalArticle

238 Citations (Scopus)

Abstract

Previous studies have shown that transection of the sciatic nerve induces dramatic changes in sodium currents of axotomized dorsal root ganglion (DRG) neurons, which are paralleled by significant changes in the levels of transcripts of several sodium channels expressed in these neurons. Sodium currents that are resistant to tetrodotoxin (TTX-R) and the transcripts of two TTX-R sodium channels are significantly attenuated, while a rapidly repriming tetrodotoxin-sensitive (TTX-S) current emerges and the transcripts of α-III sodium channel, which produce a TTX-S current when expressed in oocytes, are up-regulated. We report here on changes in sodium currents and sodium channel transcripts in DRG neurons in the chronic constriction injury (CCI) model of neuropathic pain. CCI-induced changes in DRG neurons, 14 days post-surgery, mirror those of axotomy. Transcripts of NaN and SNS, two sensory neuron-specific TTX-R sodium channels, are significantly down-regulated as is the TTX-R sodium current, while transcripts of the TTX-S α-III sodium channel and a rapidly repriming TTX-S Na current are up-regulated in small diameter DRG neurons. These changes may provide at least a partial basis for the hyperexcitablity of DRG neurons that contributes to hyperalgesia in this model.

Original languageEnglish (US)
Pages (from-to)591-600
Number of pages10
JournalPain
Volume83
Issue number3
DOIs
StatePublished - Dec 1 1999
Externally publishedYes

Fingerprint

Sodium Channels
Spinal Ganglia
Neuralgia
Constriction
Tetrodotoxin
Neurons
Wounds and Injuries
Sodium
Axotomy
Hyperalgesia
Sensory Receptor Cells
Sciatic Nerve
Ambulatory Surgical Procedures
Oocytes

Keywords

  • In situ hybridization
  • Rapidly repriming Na current
  • RT- PCR
  • Thermal hyperalgesia
  • TTX-R Na current

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Neuroscience(all)
  • Pharmacology
  • Clinical Psychology

Cite this

Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain. / Dib-Hajj, Sulayman D.; Fjell, Jenny; Cummins, Theodore; Zheng, Zheng; Fried, Kaj; Lamotte, Robert; Black, Joel A.; Waxman, Stephen G.

In: Pain, Vol. 83, No. 3, 01.12.1999, p. 591-600.

Research output: Contribution to journalArticle

Dib-Hajj, SD, Fjell, J, Cummins, T, Zheng, Z, Fried, K, Lamotte, R, Black, JA & Waxman, SG 1999, 'Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain', Pain, vol. 83, no. 3, pp. 591-600. https://doi.org/10.1016/S0304-3959(99)00169-4
Dib-Hajj, Sulayman D. ; Fjell, Jenny ; Cummins, Theodore ; Zheng, Zheng ; Fried, Kaj ; Lamotte, Robert ; Black, Joel A. ; Waxman, Stephen G. / Plasticity of sodium channel expression in DRG neurons in the chronic constriction injury model of neuropathic pain. In: Pain. 1999 ; Vol. 83, No. 3. pp. 591-600.
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