Platelet-activating factor and solute transport processes in the kidney

Rajash Handa, Jack W. Strandhoy, Carlos E. Giammattei, Shelly E. Handa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We examined the hemodynamic and tubular transport mechanisms by which platelet-activating factor (PAF) regulates salt and water excretion. In anesthetized, renally denervated male Wistar rats, with raised systemic blood pressure and renal arterial blood pressure maintained at normal levels, intrarenal PAF infusion at 2.5 ng·min-10*middot;kg-1 resulted in a small fall in systemic blood pressure (no change in renal arterial blood pressure) and an increase in renal blood flow and urinary water, sodium, and potassium excretion rates. The PAF-induced changes in cardiovascular and renal hemodynamic function were abolished and renal excretory function greatly attenuated by treating rats with a nitric oxide synthase inhibitor. To determine whether a tubular site of action was involved in the natriuretic effect of PAF, cortical proximal tubules were enzymatically dissociated from male Wistar rat kidneys, and oxygen consumption rates (Qo2) were used as an integrated index of transcellular sodium transport. PAF at 1 nM maximally inhibited Qo2 in both untreated and nystatin-stimulated (sodium entry into renal cell is not rate limiting) proximal tubules by ∼20%. Blockade of PAF receptors or Na+-K+-ATPase pump activity with BN-52021 or ouabain, respectively, abolished the effect of PAF on nystatin-stimulated proximal tubule Qo2. Inhibition of nitric oxide synthase or guanylate cyclase systems did not alter PAF-mediated inhibition of nystatin-stimulated proximal tubule Qo2, whereas phospholipase A2 or cytochrome-P-450 monooxygenase inhibition resulted in a 40-60% reduction. These findings suggest that stimulation of PAF receptors on the proximal tubule decreases transcellular sodium transport by activating phospholipase A2 and the cytochrome-P-450 monooxygenase pathways that lead to the inhibition of an ouabain-sensitive component of the basolateral Na+-K+-ATPase pump. Thus PAF can activate both an arachidonate pathway-mediated suppression of proximal tubule sodium transport and a nitric oxide pathway-mediated dilatory action on renal hemodynamics that likely contributes to the natriuresis and diuresis observed in vivo.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume284
Issue number2 53-2
StatePublished - Feb 1 2003
Externally publishedYes

Fingerprint

Platelet Activating Factor
Kidney
Nystatin
Sodium
Transcytosis
ginkgolide B
Hemodynamics
Phospholipases A2
Ouabain
Nitric Oxide Synthase
Cytochrome P-450 Enzyme System
Wistar Rats
Arterial Pressure
Blood Pressure
Natriuretic Agents
Natriuresis
Water
Renal Circulation
Guanylate Cyclase
Diuresis

Keywords

  • Blood pressure
  • Cytochrome P-450 monooxygenase
  • Electrolyte excretion
  • Guanylate cyclase
  • Nitric oxide
  • Phospholipase A
  • Proximal tubule
  • Renal blood flow
  • Sodium-potassium-adenosine 5′-triphosphatase
  • Urinary water
  • Vasopressin

ASJC Scopus subject areas

  • Physiology

Cite this

Platelet-activating factor and solute transport processes in the kidney. / Handa, Rajash; Strandhoy, Jack W.; Giammattei, Carlos E.; Handa, Shelly E.

In: American Journal of Physiology - Renal Physiology, Vol. 284, No. 2 53-2, 01.02.2003.

Research output: Contribution to journalArticle

Handa, Rajash ; Strandhoy, Jack W. ; Giammattei, Carlos E. ; Handa, Shelly E. / Platelet-activating factor and solute transport processes in the kidney. In: American Journal of Physiology - Renal Physiology. 2003 ; Vol. 284, No. 2 53-2.
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