Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway

J. G. Hu, S. L. Fu, Y. X. Wang, Y. Li, X. Y. Jiang, X. F. Wang, M. S. Qiu, P. H. Lu, Xiao-Ming Xu

Research output: Contribution to journalArticle

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Abstract

Platelet-derived growth factor-AA (PDGF-AA) has been used as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). Whether it plays a role in oligodendrocyte lineage differentiation of neural stem cells (NSCs) is unclear. Here we report that PDGF-AA is an instructional signal required for the differentiation of embryonic forebrain NSCs into O4-positive oligodendrocytes. Moreover, such PDGF-AA-induced oligodendrocyte differentiation appears to be mediated by the extracellular signal-regulated kinases 1 and 2 (Erk1/2) but not phosphatidylinositol-3 kinase (PI3K) pathway. Finally, PDGF-AA treatment resulted in a significant increase in the expression of the oligodendrocyte-specific transcriptional factor Olig2 in an Erk1/2-dependent mechanism at early stages of oligodendrogliogenesis. Together, our studies provide cellular and molecular evidence to suggest that PDGF-AA is a key molecule that regulates the differentiation of embryonic NSCs into oligodendrocytes. The action of PDGF-AA is mediated by the activation of Erk pathway which involves the downstream upregulation of transcriptional factor Olig2.

Original languageEnglish
Pages (from-to)138-147
Number of pages10
JournalNeuroscience
Volume151
Issue number1
DOIs
StatePublished - Jan 2 2008

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Platelet-Derived Growth Factor
Extracellular Signal-Regulated MAP Kinases
Oligodendroglia
Neural Stem Cells
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinase 1
Phosphatidylinositol 3-Kinase
Embryonic Stem Cells
Prosencephalon
Mitogens
Up-Regulation
Stem Cells

Keywords

  • differentiation
  • Erk1/2
  • neural stem cells
  • Olig2
  • oligodendrocytes
  • PDGF-AA

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway. / Hu, J. G.; Fu, S. L.; Wang, Y. X.; Li, Y.; Jiang, X. Y.; Wang, X. F.; Qiu, M. S.; Lu, P. H.; Xu, Xiao-Ming.

In: Neuroscience, Vol. 151, No. 1, 02.01.2008, p. 138-147.

Research output: Contribution to journalArticle

Hu, J. G. ; Fu, S. L. ; Wang, Y. X. ; Li, Y. ; Jiang, X. Y. ; Wang, X. F. ; Qiu, M. S. ; Lu, P. H. ; Xu, Xiao-Ming. / Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway. In: Neuroscience. 2008 ; Vol. 151, No. 1. pp. 138-147.
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