Platelet-derived growth factor-AA mediates oligodendrocyte lineage differentiation through activation of extracellular signal-regulated kinase signaling pathway

J. G. Hu, S. L. Fu, Y. X. Wang, Y. Li, X. Y. Jiang, X. F. Wang, M. S. Qiu, P. H. Lu, X. M. Xu

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

Platelet-derived growth factor-AA (PDGF-AA) has been used as a potent mitogen for the proliferation of oligodendrocyte progenitor cells (OPCs). Whether it plays a role in oligodendrocyte lineage differentiation of neural stem cells (NSCs) is unclear. Here we report that PDGF-AA is an instructional signal required for the differentiation of embryonic forebrain NSCs into O4-positive oligodendrocytes. Moreover, such PDGF-AA-induced oligodendrocyte differentiation appears to be mediated by the extracellular signal-regulated kinases 1 and 2 (Erk1/2) but not phosphatidylinositol-3 kinase (PI3K) pathway. Finally, PDGF-AA treatment resulted in a significant increase in the expression of the oligodendrocyte-specific transcriptional factor Olig2 in an Erk1/2-dependent mechanism at early stages of oligodendrogliogenesis. Together, our studies provide cellular and molecular evidence to suggest that PDGF-AA is a key molecule that regulates the differentiation of embryonic NSCs into oligodendrocytes. The action of PDGF-AA is mediated by the activation of Erk pathway which involves the downstream upregulation of transcriptional factor Olig2.

Original languageEnglish (US)
Pages (from-to)138-147
Number of pages10
JournalNeuroscience
Volume151
Issue number1
DOIs
StatePublished - Jan 2 2008

Keywords

  • differentiation
  • Erk1/2
  • neural stem cells
  • Olig2
  • oligodendrocytes
  • PDGF-AA

ASJC Scopus subject areas

  • Neuroscience(all)

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