Platelet-derived growth factor induces interleukin-1 receptor gene expression in Balb/c 3T3 fibroblasts

W. J. Chiou, P. D. Bonin, P. K W Harris, D. B. Carter, J. P. Singh

Research output: Contribution to journalArticle

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Abstract

Our previous studies have demonstrated that platelet-derived growth factor (PDGF) modulated cellular responses to interleukin-1 (IL-1). In this communication, we show that PDGF regulates expression of IL-1 receptor (IL-1 R) gene. Treatment of quiescent cultures of Balb/c 3T3 fibroblasts with PDGF produced 20-30-fold stimulation of IL-1 R mRNA with a concomitant increase in cell surface 125I-binding. IL-1 R mRNA accumulation occurred after an initial lag period and with a time course preceding the increase in 125I-IL-1 binding to cells. Induction of IL-1 R mRNA was blocked by inhibitors of protein synthesis, suggesting that a product of a gene expressed immediately after PDGF addition is required for IL-1 R gene expression. These latter data provide evidence for an ordered sequence of expression of PDGF-inducible 'immediate early' gene(s) and IL-1 R gene. These results suggest that in connective tissues, PDGF may be an important determinant in initiating and maintaining cellular responses to IL-1. Such responses may have important consequences in the actions of IL-1 under normal and pathological conditions such as arthritis and atherosclerosis.

Original languageEnglish (US)
Pages (from-to)21442-21445
Number of pages4
JournalJournal of Biological Chemistry
Volume264
Issue number36
StatePublished - 1989
Externally publishedYes

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Interleukin-1 Receptors
Platelet-Derived Growth Factor
Fibroblasts
Interleukin-1
Gene expression
Gene Expression
Genes
vpr Genes
Messenger RNA
Protein Synthesis Inhibitors
Immediate-Early Genes
Cell culture
Connective Tissue
Arthritis
Atherosclerosis
Tissue
Communication

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chiou, W. J., Bonin, P. D., Harris, P. K. W., Carter, D. B., & Singh, J. P. (1989). Platelet-derived growth factor induces interleukin-1 receptor gene expression in Balb/c 3T3 fibroblasts. Journal of Biological Chemistry, 264(36), 21442-21445.

Platelet-derived growth factor induces interleukin-1 receptor gene expression in Balb/c 3T3 fibroblasts. / Chiou, W. J.; Bonin, P. D.; Harris, P. K W; Carter, D. B.; Singh, J. P.

In: Journal of Biological Chemistry, Vol. 264, No. 36, 1989, p. 21442-21445.

Research output: Contribution to journalArticle

Chiou, WJ, Bonin, PD, Harris, PKW, Carter, DB & Singh, JP 1989, 'Platelet-derived growth factor induces interleukin-1 receptor gene expression in Balb/c 3T3 fibroblasts', Journal of Biological Chemistry, vol. 264, no. 36, pp. 21442-21445.
Chiou, W. J. ; Bonin, P. D. ; Harris, P. K W ; Carter, D. B. ; Singh, J. P. / Platelet-derived growth factor induces interleukin-1 receptor gene expression in Balb/c 3T3 fibroblasts. In: Journal of Biological Chemistry. 1989 ; Vol. 264, No. 36. pp. 21442-21445.
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