Platelet-derived growth factor induces proliferation of hyperplastic human prostatic stromal cells

C. J. Vlahos, T. D. Kriauciunas, P. E. Gleason, J. A. Jones, John Eble, D. Salvas, J. F. Falcone, K. S. Hirsch

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Prostatic hyperplasia (BPH) is a very common disease in elderly men and is characterized by abnormal proliferation of the stromal and epithelial cells of the prostate. The observation that BPH often occurs in association with chronic inflammation has led to the examination of the possibility that platelet-derived growth factor (PDGF), which is released in response to inflammation, may be an etiological factor in the genesis of the disease. It has been shown that cultured cells derived from human prostatic tissue express high affinity PDGF-β receptors based on receptor binding and cross-linking studies with [125I]-PDGF-BB. The experiments presented below demonstrate that PDGF receptors are activated in response to the growth factor and that mitogenesis is induced. PDGF-BB treatment of cultured human prostate cells derived from patients with BPH activates the signal transduction pathway of the PDGF receptor as shown by the presence of several phosphoproteins in antiphosphotyrosine immunoprecipitates, including autophosphorylation of the PDGF receptor. Phosphatidylinositol (PI) 3-kinase activity is also increased in cells stimulated with PDGF. The addition of PDGF-BB to the medium causes a variable but dose-dependent increase in [3H]-thymidine incorporation. This paper describes the first demonstration that PDGF is a potent mitogen for human cells derived from patients exhibiting prostatic hyperplasia, and also demonstrates that the cellular response to PDGF-BB is heterogeneous in a manner that is consistent with the varying degree of hyperplasia and inflammation clinically and histologically in the tissue specimens.

Original languageEnglish (US)
Pages (from-to)404-413
Number of pages10
JournalJournal of Cellular Biochemistry
Volume52
Issue number4
StatePublished - 1993

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Platelet-Derived Growth Factor Receptors
Platelet-Derived Growth Factor
Stromal Cells
Prostatic Hyperplasia
Inflammation
Prostate
Cells
Phosphatidylinositol 3-Kinase
Tissue
Signal transduction
Phosphoproteins
Mitogens
Thymidine
Hyperplasia
Cultured Cells
Signal Transduction
Intercellular Signaling Peptides and Proteins
Demonstrations
Epithelial Cells
Association reactions

Keywords

  • inflammation
  • mitogen
  • protein phosphorylation
  • signal transduction
  • tyrosine kinase

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Vlahos, C. J., Kriauciunas, T. D., Gleason, P. E., Jones, J. A., Eble, J., Salvas, D., ... Hirsch, K. S. (1993). Platelet-derived growth factor induces proliferation of hyperplastic human prostatic stromal cells. Journal of Cellular Biochemistry, 52(4), 404-413.

Platelet-derived growth factor induces proliferation of hyperplastic human prostatic stromal cells. / Vlahos, C. J.; Kriauciunas, T. D.; Gleason, P. E.; Jones, J. A.; Eble, John; Salvas, D.; Falcone, J. F.; Hirsch, K. S.

In: Journal of Cellular Biochemistry, Vol. 52, No. 4, 1993, p. 404-413.

Research output: Contribution to journalArticle

Vlahos, CJ, Kriauciunas, TD, Gleason, PE, Jones, JA, Eble, J, Salvas, D, Falcone, JF & Hirsch, KS 1993, 'Platelet-derived growth factor induces proliferation of hyperplastic human prostatic stromal cells', Journal of Cellular Biochemistry, vol. 52, no. 4, pp. 404-413.
Vlahos, C. J. ; Kriauciunas, T. D. ; Gleason, P. E. ; Jones, J. A. ; Eble, John ; Salvas, D. ; Falcone, J. F. ; Hirsch, K. S. / Platelet-derived growth factor induces proliferation of hyperplastic human prostatic stromal cells. In: Journal of Cellular Biochemistry. 1993 ; Vol. 52, No. 4. pp. 404-413.
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