Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction

A pilot investigation

David C. Calverley, Ivan P. Casserly, Qamrul G. Choudhury, Tzu L. Phang, Bifeng Gao, John C. Messenger, Mark W. Geraci

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Platelets play a major role in the pathophysiology of acute myocardial infarction (AMI). Recent evidence reveals megakaryocyte- derived platelet pre-mRNA is spliced to mRNA and then translated into functional proteins in response to external stimulation. An exon microarray analyzes pre-mRNA alternative splicing and is thus applicable for studying gene expression in the anucleate platelet. We hypothesized a subset of megakaryocyte/platelet genes exists that are significantly over or underexpressed in AMI compared with stable coronary artery disease (CAD), yielding a gene expression profile for further study. Microarray analysis employing platelet mRNA was used to generate gene expression data in the above two patient groups. Unsupervised hierarchical clustering has revealed an expression profile that includes 95 over- or under-expressed genes depicted in a heat map where separation of both sets takes place. This preliminary study reveals a platelet-based gene expression signature that differentiates between AMI and stable CAD, and further study may yield a prognostic tool for a future AMI event in atherosclerosis risk factor-based subsets of CAD patients.

Original languageEnglish (US)
Pages (from-to)9-15
Number of pages7
JournalClinical Medicine Insights: Blood Disorders
Volume3
StatePublished - 2010
Externally publishedYes

Fingerprint

Blood Platelets
Biomarkers
Myocardial Infarction
Gene Expression
Coronary Artery Disease
Megakaryocytes
RNA Precursors
Microarray Analysis
Transcriptome
Messenger RNA
Alternative Splicing
Genes
Cluster Analysis
Exons
Atherosclerosis
Hot Temperature
Proteins

Keywords

  • Gene expression
  • Myocardial infarction
  • Platelets
  • Thrombosis

ASJC Scopus subject areas

  • Hematology

Cite this

Calverley, D. C., Casserly, I. P., Choudhury, Q. G., Phang, T. L., Gao, B., Messenger, J. C., & Geraci, M. W. (2010). Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction: A pilot investigation. Clinical Medicine Insights: Blood Disorders, 3, 9-15.

Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction : A pilot investigation. / Calverley, David C.; Casserly, Ivan P.; Choudhury, Qamrul G.; Phang, Tzu L.; Gao, Bifeng; Messenger, John C.; Geraci, Mark W.

In: Clinical Medicine Insights: Blood Disorders, Vol. 3, 2010, p. 9-15.

Research output: Contribution to journalArticle

Calverley, DC, Casserly, IP, Choudhury, QG, Phang, TL, Gao, B, Messenger, JC & Geraci, MW 2010, 'Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction: A pilot investigation', Clinical Medicine Insights: Blood Disorders, vol. 3, pp. 9-15.
Calverley, David C. ; Casserly, Ivan P. ; Choudhury, Qamrul G. ; Phang, Tzu L. ; Gao, Bifeng ; Messenger, John C. ; Geraci, Mark W. / Platelet gene expression as a biomarker risk stratification tool in acute myocardial infarction : A pilot investigation. In: Clinical Medicine Insights: Blood Disorders. 2010 ; Vol. 3. pp. 9-15.
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