Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth

Daotai Nie, Gilda G. Hillman, Timothy Geddes, Keqin Tang, Christopher Pierson, David J. Grignon, Kenneth V. Honn

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164 Scopus citations

Abstract

Previously, we found a positive correlation between the expression of platelet-type 12-lipoxygenase (12-LOX) and the progression of human prostate adenocarcinoma (PCa; Gao et al., Urology, 46: 227-237, 1995). To determine the role of 12-LOX in PCa progression, we generated stable 12-LOX-transfected PC3 cells, which synthesize high levels of 12-LOX protein and 12(S)- hydroxyeicosatetraenoic acid metabolite. In vitro, 12 LOX-transfected PC3 cells demonstrated a proliferation rate similar to neo controls. However, following s.c. injection into athymic nude mice, 12-LOX-transfected PC3 cells formed larger tumors than did the controls. Decreased necrosis and increased vascularization were observed in the tumors from 12-LOX-transfected PC3 cells. Both endothelial cell migration and Matrigel implantation assays indicate that 12-LOX-transfected PC3 cells were more angiogenic than their neo controls. These data indicate that 12-LOX stimulates human PCa tumor growth by a novel angiogenic mechanism.

Original languageEnglish (US)
Pages (from-to)4047-4051
Number of pages5
JournalCancer Research
Volume58
Issue number18
StatePublished - Sep 15 1998

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ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Nie, D., Hillman, G. G., Geddes, T., Tang, K., Pierson, C., Grignon, D. J., & Honn, K. V. (1998). Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth. Cancer Research, 58(18), 4047-4051.