Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth

Daotai Nie, Gilda G. Hillman, Timothy Geddes, Keqin Tang, Christopher Pierson, David Grignon, Kenneth V. Honn

Research output: Contribution to journalArticle

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Abstract

Previously, we found a positive correlation between the expression of platelet-type 12-lipoxygenase (12-LOX) and the progression of human prostate adenocarcinoma (PCa; Gao et al., Urology, 46: 227-237, 1995). To determine the role of 12-LOX in PCa progression, we generated stable 12-LOX-transfected PC3 cells, which synthesize high levels of 12-LOX protein and 12(S)- hydroxyeicosatetraenoic acid metabolite. In vitro, 12 LOX-transfected PC3 cells demonstrated a proliferation rate similar to neo controls. However, following s.c. injection into athymic nude mice, 12-LOX-transfected PC3 cells formed larger tumors than did the controls. Decreased necrosis and increased vascularization were observed in the tumors from 12-LOX-transfected PC3 cells. Both endothelial cell migration and Matrigel implantation assays indicate that 12-LOX-transfected PC3 cells were more angiogenic than their neo controls. These data indicate that 12-LOX stimulates human PCa tumor growth by a novel angiogenic mechanism.

Original languageEnglish (US)
Pages (from-to)4047-4051
Number of pages5
JournalCancer Research
Volume58
Issue number18
StatePublished - Sep 15 1998
Externally publishedYes

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Arachidonate 12-Lipoxygenase
Prostate
Blood Platelets
Carcinoma
Growth
Neoplasms
Nude Mice
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Protein S
Urology
Cell Movement
Adenocarcinoma
Necrosis
Endothelial Cells
Injections

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nie, D., Hillman, G. G., Geddes, T., Tang, K., Pierson, C., Grignon, D., & Honn, K. V. (1998). Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth. Cancer Research, 58(18), 4047-4051.

Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth. / Nie, Daotai; Hillman, Gilda G.; Geddes, Timothy; Tang, Keqin; Pierson, Christopher; Grignon, David; Honn, Kenneth V.

In: Cancer Research, Vol. 58, No. 18, 15.09.1998, p. 4047-4051.

Research output: Contribution to journalArticle

Nie, D, Hillman, GG, Geddes, T, Tang, K, Pierson, C, Grignon, D & Honn, KV 1998, 'Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth', Cancer Research, vol. 58, no. 18, pp. 4047-4051.
Nie D, Hillman GG, Geddes T, Tang K, Pierson C, Grignon D et al. Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth. Cancer Research. 1998 Sep 15;58(18):4047-4051.
Nie, Daotai ; Hillman, Gilda G. ; Geddes, Timothy ; Tang, Keqin ; Pierson, Christopher ; Grignon, David ; Honn, Kenneth V. / Platelet-type 12-lipoxygenase in a human prostate carcinoma stimulates angiogenesis and tumor growth. In: Cancer Research. 1998 ; Vol. 58, No. 18. pp. 4047-4051.
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