Platelets from diabetic pigs exhibit hypersensitivity to thrombin

Shivendra D. Shukla, Sanjay V. Kansra, M. A. Reddy, Sundeep M. Shukla, David M. Klachko, Michael Sturek

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Responses of platelets from diabetic and diabetic-hyperlipidemic pigs were studied. Pigs were made diabetic with single dose of alloxan, which acts by selectively destroying insulin-producing pancreatic β cells thus inducing type 1 diabetes. Pigs were kept for 1 or 12 wk, during which thrombin-induced aggregation was monitored in washed platelets. The platelets showed increased sensitivity to aggregation within 1 wk of induction of diabetes. Hyperlipidemia alone for 12 wk did not increase platelet hypersensitivity, but hyperlipidemia together with diabetes significantly increased thrombin-induced platelet aggregation. Because this hypersensitivity occurred in washed platelets, this characteristic appears to be independent of any contribution by plasma factors or other blood cells. The hypersensitivity of platelets from diabetic pigs correlated with decreased activity of mitogen-activated protein kinase. These studies offer the first evidence that platelet hyperactivity occurs during the early stages (within a week) of induction of diabetes in pigs and before manifestation of other cardiovascular problems.

Original languageEnglish (US)
Pages (from-to)481-484
Number of pages4
JournalComparative medicine
Volume58
Issue number5
StatePublished - Oct 1 2008

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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    Shukla, S. D., Kansra, S. V., Reddy, M. A., Shukla, S. M., Klachko, D. M., & Sturek, M. (2008). Platelets from diabetic pigs exhibit hypersensitivity to thrombin. Comparative medicine, 58(5), 481-484.