Polyclonal activation of immunoglobulin secretion in B lymphocytes induced by staphylococcal peptidoglycan

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Staphylococcal peptidoglycan (PG) is a B cell mitogen and immunomodulator in mice. The ability of PG to induce the secretion of polyclonal antibodies in murine lymphocyte cultures was studied using the protein A hemolytic plaque assay. PG was as effective as or more effective than lipopolysaccharide as an inducer of polyclonal antibodies in spleen, lymph node, and bone marrow lymphocytes. The highest numbers of immunoglobulin (Ig) secreting cells were induced in the spleen, and the lowest in the bone marrow cell cultures. All major classes of Ig (IgM, IgG, and IgA) were induced, and the maximal numbers of Ig-secreting cells were detected between days 4 and 5 of culture. The induction of Ig secretion by PG was T cell, 2-mercaptoethanol, and to a large extent macrophage independent.

Original languageEnglish (US)
Pages (from-to)2478-2483
Number of pages6
JournalJournal of Immunology
Volume125
Issue number6
StatePublished - 1980
Externally publishedYes

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Peptidoglycan
Immunoglobulins
B-Lymphocytes
Antibody-Producing Cells
Spleen
Lymphocytes
Mercaptoethanol
Antibodies
Immunoglobulin Isotypes
Staphylococcal Protein A
Immunologic Factors
Mitogens
Bone Marrow Cells
Immunoglobulin A
Immunoglobulin M
Lipopolysaccharides
Cell Culture Techniques
Immunoglobulin G
Lymph Nodes
Bone Marrow

ASJC Scopus subject areas

  • Immunology

Cite this

Polyclonal activation of immunoglobulin secretion in B lymphocytes induced by staphylococcal peptidoglycan. / Dziarski, Roman.

In: Journal of Immunology, Vol. 125, No. 6, 1980, p. 2478-2483.

Research output: Contribution to journalArticle

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