Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes

P. Rotwein, R. Chyn, John Chirgwin, B. Cordell, H. M. Goodman, M. A. Permut

Research output: Contribution to journalArticle

Abstract

The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P

Original languageEnglish (US)
Pages (from-to)1117-1120
Number of pages4
JournalScience
Volume213
Issue number4512
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

5' Flanking Region
Type 2 Diabetes Mellitus
Insulin
Transcription Initiation Site
DNA
Southern Blotting
Genes

ASJC Scopus subject areas

  • General

Cite this

Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes. / Rotwein, P.; Chyn, R.; Chirgwin, John; Cordell, B.; Goodman, H. M.; Permut, M. A.

In: Science, Vol. 213, No. 4512, 1981, p. 1117-1120.

Research output: Contribution to journalArticle

Rotwein, P. ; Chyn, R. ; Chirgwin, John ; Cordell, B. ; Goodman, H. M. ; Permut, M. A. / Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes. In: Science. 1981 ; Vol. 213, No. 4512. pp. 1117-1120.
@article{d1fcd239ce564b82a2ae086490a26808,
title = "Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes",
abstract = "The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P",
author = "P. Rotwein and R. Chyn and John Chirgwin and B. Cordell and Goodman, {H. M.} and Permut, {M. A.}",
year = "1981",
doi = "10.1126/science.6267694",
language = "English (US)",
volume = "213",
pages = "1117--1120",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "4512",

}

TY - JOUR

T1 - Polymorphism in the 5'-flanking region of the human insulin gene and its possible relation to type 2 diabetes

AU - Rotwein, P.

AU - Chyn, R.

AU - Chirgwin, John

AU - Cordell, B.

AU - Goodman, H. M.

AU - Permut, M. A.

PY - 1981

Y1 - 1981

N2 - The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P

AB - The arrangement of the human insulin gene in DNA from 87 individuals was analyzed by the Southern blot hybridization technique with a cloned genomic human insulin probe. Insertions of 1.5 to 3.4 kilobase pairs in the 5'-flanking region of the gene were found in DNA from 38 individuals. These insertions occurred within 1.3 kilobase pairs of the transcription initiation site. In contrast, no insertions were observed in the region 3' to the coding sequence. The prevalence of these insertions in type 2 diabetes was significantly greater than in the other groups (P

UR - http://www.scopus.com/inward/record.url?scp=0019855977&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019855977&partnerID=8YFLogxK

U2 - 10.1126/science.6267694

DO - 10.1126/science.6267694

M3 - Article

VL - 213

SP - 1117

EP - 1120

JO - Science

JF - Science

SN - 0036-8075

IS - 4512

ER -