Polymorphisms of xenobiotic metabolizing genes in oropharyngeal carcinoma

Armando G. Amador, Paul D. Righi, Shokri Radpour, Eric T. Everett, Edward Weisberger, Mark Langer, George J. Eckert, Arden G. Christen, Samuel Campbell, Don Jon Summerlin, Nichole Reynolds, James K. Hartsfield

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective. The objective was to determine the prevalence of the polymorphisms of the microsomal epoxide hydrolase (Ephx1), glutathione S-transferase μ1 (GSTM1), 01 (GSTT1), and π1 (GSTP1) genes in patients with oropharyngeal carcinoma. Study design. Gene polymorphisms in 137 patients with oropharyngeal carcinoma were determined by polymerase chain reaction and restriction enzyme digestion for xenobiotic metabolizing enzymes that have been implicated in the carcinogenesis of tobacco-related neoplasias and compared with a population sample of 99 persons. Results. At Ephx1 (microsomal epoxide hydrolase) codon 113, an overrepresentation of the greater activity genotype (Tyr/Tyr) was observed for male ever-smokers alone, both male and female ever-smokers, female never-smokers alone, and in both male and female never-smokers, compared with a control population sample. At codon 139, Ephx1 showed no differences. There was an overrepresentation of homozygosity for the GSTT1 (glutathione S-transferase θ1) null allele [but not for the GSTM1 (glutathione S-transferase μ1) null allele] in ever-smokers, when compared with controls. Polymorphisms at the GSTP1 (glutathione S-transferase π1) locus did not show differences versus controls, although in the never-smoker cancer sample there was a higher prevalence of the B/B genotype compared with ever-smokers. Conclusion. The Ephx1 codon 113 Tyr/Tyr variant, as well as homozygosity for the GSTT1 null allele, is associated with oropharyngeal carcinogenesis.

Original languageEnglish
Pages (from-to)440-445
Number of pages6
JournalOral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
Volume93
Issue number4
DOIs
StatePublished - 2002

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Xenobiotics
Glutathione Transferase
Carcinoma
Codon
Genes
Epoxide Hydrolases
Alleles
Carcinogenesis
Genotype
Enzymes
Population
Tobacco
Digestion
Neoplasms
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Dentistry(all)
  • Pathology and Forensic Medicine
  • Radiology Nuclear Medicine and imaging
  • Surgery

Cite this

Polymorphisms of xenobiotic metabolizing genes in oropharyngeal carcinoma. / Amador, Armando G.; Righi, Paul D.; Radpour, Shokri; Everett, Eric T.; Weisberger, Edward; Langer, Mark; Eckert, George J.; Christen, Arden G.; Campbell, Samuel; Summerlin, Don Jon; Reynolds, Nichole; Hartsfield, James K.

In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, Vol. 93, No. 4, 2002, p. 440-445.

Research output: Contribution to journalArticle

Amador, AG, Righi, PD, Radpour, S, Everett, ET, Weisberger, E, Langer, M, Eckert, GJ, Christen, AG, Campbell, S, Summerlin, DJ, Reynolds, N & Hartsfield, JK 2002, 'Polymorphisms of xenobiotic metabolizing genes in oropharyngeal carcinoma', Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, vol. 93, no. 4, pp. 440-445. https://doi.org/10.1067/moe.2002.122586
Amador, Armando G. ; Righi, Paul D. ; Radpour, Shokri ; Everett, Eric T. ; Weisberger, Edward ; Langer, Mark ; Eckert, George J. ; Christen, Arden G. ; Campbell, Samuel ; Summerlin, Don Jon ; Reynolds, Nichole ; Hartsfield, James K. / Polymorphisms of xenobiotic metabolizing genes in oropharyngeal carcinoma. In: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics. 2002 ; Vol. 93, No. 4. pp. 440-445.
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abstract = "Objective. The objective was to determine the prevalence of the polymorphisms of the microsomal epoxide hydrolase (Ephx1), glutathione S-transferase μ1 (GSTM1), 01 (GSTT1), and π1 (GSTP1) genes in patients with oropharyngeal carcinoma. Study design. Gene polymorphisms in 137 patients with oropharyngeal carcinoma were determined by polymerase chain reaction and restriction enzyme digestion for xenobiotic metabolizing enzymes that have been implicated in the carcinogenesis of tobacco-related neoplasias and compared with a population sample of 99 persons. Results. At Ephx1 (microsomal epoxide hydrolase) codon 113, an overrepresentation of the greater activity genotype (Tyr/Tyr) was observed for male ever-smokers alone, both male and female ever-smokers, female never-smokers alone, and in both male and female never-smokers, compared with a control population sample. At codon 139, Ephx1 showed no differences. There was an overrepresentation of homozygosity for the GSTT1 (glutathione S-transferase θ1) null allele [but not for the GSTM1 (glutathione S-transferase μ1) null allele] in ever-smokers, when compared with controls. Polymorphisms at the GSTP1 (glutathione S-transferase π1) locus did not show differences versus controls, although in the never-smoker cancer sample there was a higher prevalence of the B/B genotype compared with ever-smokers. Conclusion. The Ephx1 codon 113 Tyr/Tyr variant, as well as homozygosity for the GSTT1 null allele, is associated with oropharyngeal carcinogenesis.",
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AU - Weisberger, Edward

AU - Langer, Mark

AU - Eckert, George J.

AU - Christen, Arden G.

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AU - Summerlin, Don Jon

AU - Reynolds, Nichole

AU - Hartsfield, James K.

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