Polyomavirus large T can support DNA replication in human cells

Francis Sverdrup, Brian S. Schaffhausen, Elliot J. Androphy

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Human cells are generally thought to be nonpermissive for polyomavirus (Py) DNA replication. Using transient transfection, we show that Py large T- antigen (LT) was able to support replication of a Py origin-containing plasmid in two human cell lines. Replication supported by LT in human cells was specific for the Py origin and required its enhancer sequences, as well as the previously reported critical phosphorylation sites within LT. Py replication efficiency was comparable to that of papillomavirus E1- and E2- activated DNA replication in transient assays performed in human 293 and C- 33A cells. Previous analysis of DNA replication in vitro has pointed to polymerase α-primase as a specificity determinant for polyomavirus. The data presented here imply that in certain cellular environments, Py LT must functionally interact with human polymerase α-primase to permit DNA replication.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalVirology
Volume240
Issue number1
DOIs
StatePublished - Jan 5 1998
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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