Population and conservation genetics of Crawfish Frogs, Lithobates areolatus, at their northeastern range limit

Schyler O. Nunziata, Michael Lannoo, Joseph R. Robb, Daryl R. Karns, Stacey L. Lance, Stephen C. Richter

Research output: Contribution to journalArticle

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Abstract

Crawfish Frogs (Lithobates areolatus) are a North American ranid, considered near threatened globally with populations in decline throughout their range. We studied populations of Crawfish Frogs on local and regional scales at their northeastern range limit to (1) assess the level of genetic diversity within populations, (2) estimate fine-scale genetic structure, and (3) estimate genetic differentiation between populations at the regional level. We used 10 microsatellite loci to genotype frogs collected from three regional sites in Indiana separated by 50-172 km and at one of these sites within a network of three breeding ponds <1 km apart. Heterozygosity estimates revealed high levels of diversity within these populations (mean HO: 0.54-0.67 per site), which is encouraging for future management. The degree of population subdivision was low at the regional level (FST = 0.071 for sites within 172 km). Genetic differentiation was related to geographic distance between sampling sites, as predicted by an isolation-by-distance model. We observed no genetic differentiation between individuals sampled from ponds approximately 250 m apart and slight divergence of individuals from a pond approximately 750 m away. This suggests ponds within 1 km form a genetically distinct single breeding unit composed of multiple subpopulations. Finally, we observed high genetic differentiation between southwest and southeast Indiana sites indicating historical (rather than recent) isolation of these sites. These data will be applied to a regional management plan in an attempt to recover Crawfish Frogs along the northeastern extreme of their range.

Original languageEnglish
Pages (from-to)361-368
Number of pages8
JournalJournal of Herpetology
Volume47
Issue number2
DOIs
StatePublished - Jun 2013

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Lithobates
conservation genetics
crayfish
genetic differentiation
frog
population genetics
frogs
pond
genetic variation
breeding
historic sites
subpopulation
heterozygosity
genetic structure
founder effect
genotype
divergence
microsatellite repeats
sampling
loci

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Ecology, Evolution, Behavior and Systematics

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Population and conservation genetics of Crawfish Frogs, Lithobates areolatus, at their northeastern range limit. / Nunziata, Schyler O.; Lannoo, Michael; Robb, Joseph R.; Karns, Daryl R.; Lance, Stacey L.; Richter, Stephen C.

In: Journal of Herpetology, Vol. 47, No. 2, 06.2013, p. 361-368.

Research output: Contribution to journalArticle

Nunziata, Schyler O. ; Lannoo, Michael ; Robb, Joseph R. ; Karns, Daryl R. ; Lance, Stacey L. ; Richter, Stephen C. / Population and conservation genetics of Crawfish Frogs, Lithobates areolatus, at their northeastern range limit. In: Journal of Herpetology. 2013 ; Vol. 47, No. 2. pp. 361-368.
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