Portal vein glucose entry triggers a coordinated cellular response that potentiates hepatic glucose uptake and storage in normal but not high-fat/high-fructose-fed dogs

Katie C. Coate, Guillaume Kraft, Jose M. Irimia, Marta S. Smith, Ben Farmer, Doss W. Neal, Peter Roach, Masakazu Shiota, Alan D. Cherrington

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The cellular events mediating the pleiotropic actions of portal vein glucose (PoG) delivery on hepatic glucose disposition have not been clearly defined. Likewise, the molecular defects associated with postprandial hyperglycemia and impaired hepatic glucose uptake (HGU) following consumption of a high-fat, highfructose diet (HFFD) are unknown. Our goal was to identify hepatocellular changes elicited by hyperinsulinemia, hyperglycemia, and PoG signaling in normal chow-fed (CTR) and HFFD-fed dogs. In CTR dogs, we demonstrated that PoG infusion in the presence of hyperinsulinemia and hyperglycemia triggered an increase in the activity of hepatic glucokinase (GK) and glycogen synthase (GS), which occurred in association with further augmentation in HGU and glycogen synthesis (GSYN) in vivo. In contrast, 4 weeks of HFFD feeding markedly reduced GK protein content and impaired the activation of GS in association with diminished HGU and GSYN in vivo. Furthermore, the enzymatic changes associated with PoG sensing in chow-fed animals were abolished in HFFD-fed animals, consistent with loss of the stimulatory effects of PoG delivery. These data reveal new insight into the molecular physiology of the portal glucose signaling mechanism under normal conditions and to the pathophysiology of aberrant postprandial hepatic glucose disposition evident under a diet-induced glucose-intolerant condition.

Original languageEnglish
Pages (from-to)392-400
Number of pages9
JournalDiabetes
Volume62
Issue number2
DOIs
StatePublished - Feb 2013

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Portal Vein
Fructose
Fats
Dogs
Glucose
Liver
High Fat Diet
Hyperglycemia
Glucokinase
Glycogen Synthase
Hyperinsulinism
Glycogen
Liver Glycogen
Diet

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Portal vein glucose entry triggers a coordinated cellular response that potentiates hepatic glucose uptake and storage in normal but not high-fat/high-fructose-fed dogs. / Coate, Katie C.; Kraft, Guillaume; Irimia, Jose M.; Smith, Marta S.; Farmer, Ben; Neal, Doss W.; Roach, Peter; Shiota, Masakazu; Cherrington, Alan D.

In: Diabetes, Vol. 62, No. 2, 02.2013, p. 392-400.

Research output: Contribution to journalArticle

Coate, Katie C. ; Kraft, Guillaume ; Irimia, Jose M. ; Smith, Marta S. ; Farmer, Ben ; Neal, Doss W. ; Roach, Peter ; Shiota, Masakazu ; Cherrington, Alan D. / Portal vein glucose entry triggers a coordinated cellular response that potentiates hepatic glucose uptake and storage in normal but not high-fat/high-fructose-fed dogs. In: Diabetes. 2013 ; Vol. 62, No. 2. pp. 392-400.
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