Positive and negative regulation of glycerol utilization by the c-di-GMP binding protein plza in borrelia burgdorferi

Jun Jie Zhang, Tong Chen, Youyun Yang, Jimei Du, Hongxia Li, Bryan Troxell, Ming He, Sebastian E. Carrasco, Mark Gomelsky, X. Yang

Research output: Contribution to journalArticle

Abstract

Borrelia burgdorferi, the causative agent of Lyme disease, encounters two disparate host environments during its enzootic life cycle, Ixodes ticks and mammalian hosts. B. burgdorferi has a small genome that encodes a streamlined cyclic dimeric GMP (c-di-GMP) signaling system comprising a single diguanylate cyclase, Rrp1, and two phosphodiesterases. This system is essential for spirochete survival in ticks, in part because it controls the expression of the glp operon involved in glycerol utilization. In this study, we showed that a B. burgdorferi c-di-GMP receptor, PlzA, functions as both a positive and a negative regulator for glp expression. Deletion of plzA or mutation in plzA that impaired c-di-GMP binding abolished glp expression. On the other hand, overexpression of plzA resulted in glp repression, which could be rescued by simultaneous overexpression of rrp1. plzA overexpression in the rrp1 mutant, which is devoid of c-di-GMP, or overexpression of a plzA mutant incapable of c-di-GMP binding further enhanced glp repression. Combined results suggest that c-di-GMP-bound PlzA functions as a positive regulator, whereas ligand-free PlzA acts as a negative regulator for glp expression. Thus, PlzA of B. burgdorferi with a streamlined c-di-GMP signaling system not only controls multiple targets, as previously envisioned, but has also evolved different modes of action. IMPORTANCE The Lyme disease pathogen, Borrelia burgdorferi, has a simple cyclic dimeric GMP (c-di-GMP) signaling system essential for adaptation of the pathogen to the complicated tick environment. The c-di-GMP effector of B. burgdorferi, PlzA, has been shown to regulate multiple cellular processes, including motility, osmolality sensing, and nutrient utilization. The findings of this study demonstrate that PlzA not only controls multiple targets but also has different functional modalities, allowing it to act as both positive and negative regulator of the glp operon expression. This work highlights how bacteria with a small genome can compensate for the limited regulatory repertoire by increasing the complexity of targets and modes of action in their regulatory proteins.

Original languageEnglish (US)
Article numbere0024318
JournalJournal of Bacteriology
Volume200
Issue number22
DOIs
StatePublished - Nov 1 2018
Externally publishedYes

Fingerprint

Borrelia burgdorferi
Cyclic GMP
Glycerol
Ticks
Lyme Disease
Operon
Genome
cyclic GMP-binding protein
Ixodes
Spirochaetales
Phosphoric Diester Hydrolases
Life Cycle Stages
Osmolar Concentration
Ligands
Bacteria
Food
Mutation

Keywords

  • Borrelia burgdorferi
  • Lyme disease
  • PlzA
  • Tick-borne pathogens

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Positive and negative regulation of glycerol utilization by the c-di-GMP binding protein plza in borrelia burgdorferi. / Zhang, Jun Jie; Chen, Tong; Yang, Youyun; Du, Jimei; Li, Hongxia; Troxell, Bryan; He, Ming; Carrasco, Sebastian E.; Gomelsky, Mark; Yang, X.

In: Journal of Bacteriology, Vol. 200, No. 22, e0024318, 01.11.2018.

Research output: Contribution to journalArticle

Zhang, JJ, Chen, T, Yang, Y, Du, J, Li, H, Troxell, B, He, M, Carrasco, SE, Gomelsky, M & Yang, X 2018, 'Positive and negative regulation of glycerol utilization by the c-di-GMP binding protein plza in borrelia burgdorferi', Journal of Bacteriology, vol. 200, no. 22, e0024318. https://doi.org/10.1128/JB.00243-18
Zhang, Jun Jie ; Chen, Tong ; Yang, Youyun ; Du, Jimei ; Li, Hongxia ; Troxell, Bryan ; He, Ming ; Carrasco, Sebastian E. ; Gomelsky, Mark ; Yang, X. / Positive and negative regulation of glycerol utilization by the c-di-GMP binding protein plza in borrelia burgdorferi. In: Journal of Bacteriology. 2018 ; Vol. 200, No. 22.
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