Post-translational regulation of the cellular levels of DAPK

Patricia Gallagher, Emily K. Blue

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Death associated protein kinase (DAPK) is a large, multi-domain ser/thr kinase whose activities converge upon multiple signaling pathways that regulate autophagy, caspase-dependent cell death, cell adhesion and migration. The cellular levels of DAPK are post-translationally regulated by the combined activities of two degradation systems, including the ubiquitin proteasome and an extra-lysosomal proteolysis pathway. At least three distinct E3 ubiquitin ligases target DAPK, including mindbomb1, the chaperone dependent ligase, CHIP (carboxy terminus of Hsp70-interacting protein) and a cullin RING ligase complex, KLHL20-Cul3-RBX1. In addition, it appears that the cellular levels of DAPK are also regulated by an extra-lysosomal protease, cathepsin B. While protein quality control and recycling clearly benefit cells by removal of misfolded or toxic proteins and recycling of their components, the finding that multiple surveillance systems target DAPK suggests that these protein degradation systems also act to fine tune DAPK expression levels in response to specific signaling pathways.

Original languageEnglish
Pages (from-to)306-315
Number of pages10
JournalApoptosis
Volume19
Issue number2
DOIs
StatePublished - Feb 2014

Fingerprint

Death-Associated Protein Kinases
Recycling
Ligases
Proteolysis
Proteins
Cullin Proteins
Degradation
Cathepsin B
Ubiquitin-Protein Ligases
Poisons
Cell adhesion
Autophagy
Cell death
Proteasome Endopeptidase Complex
Caspases
Ubiquitin
Cell Adhesion
Quality Control
Cell Movement
Quality control

Keywords

  • Apoptosis
  • Autophagy
  • Death associated protein kinase
  • Post-translational
  • Stability

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Biochemistry, medical
  • Cancer Research
  • Pharmaceutical Science
  • Pharmacology

Cite this

Post-translational regulation of the cellular levels of DAPK. / Gallagher, Patricia; Blue, Emily K.

In: Apoptosis, Vol. 19, No. 2, 02.2014, p. 306-315.

Research output: Contribution to journalArticle

Gallagher, Patricia ; Blue, Emily K. / Post-translational regulation of the cellular levels of DAPK. In: Apoptosis. 2014 ; Vol. 19, No. 2. pp. 306-315.
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