POU-domain sequences from the flatworm Dugesia tigrina

Gary W. Stuart, Zhu Zhu, Karuna Sampath, Michael W. King

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Redundant primers matching well-conserved sequences within vertebrate and invertebrate POU genes were used in the PCR (polymerase chain reaction) to detect three different POU-domain-containing genes within the genome of the flatworm, Dugesia tigrina. Two of these genes appear to be close homologues of class-III POU genes previously identified in Dugesia japonica, while the third represents a new class-III POU gene. Nucleotide sequences encoding highly related POU domains within the two species of planaria exhibited a surprisingly poor degree of shared identity. Since only class-III POU genes have been observed to date in flatworms, these genes may represent ancestral versions of the multiple classes of PO U genes that exist in vertebrates, and may play important roles in the development of metazoan nervous tissue.

Original languageEnglish (US)
Pages (from-to)299-300
Number of pages2
JournalGene
Volume161
Issue number2
DOIs
StatePublished - Aug 19 1995

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Platyhelminths
Planarians
Genes
Vertebrates
Nerve Tissue
Conserved Sequence
Invertebrates
Genome
Polymerase Chain Reaction

Keywords

  • DNA-binding protein
  • molecular evolution
  • planaria
  • transcription factor

ASJC Scopus subject areas

  • Genetics

Cite this

POU-domain sequences from the flatworm Dugesia tigrina. / Stuart, Gary W.; Zhu, Zhu; Sampath, Karuna; King, Michael W.

In: Gene, Vol. 161, No. 2, 19.08.1995, p. 299-300.

Research output: Contribution to journalArticle

Stuart, Gary W. ; Zhu, Zhu ; Sampath, Karuna ; King, Michael W. / POU-domain sequences from the flatworm Dugesia tigrina. In: Gene. 1995 ; Vol. 161, No. 2. pp. 299-300.
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