Poxvirus tropism for primary human leukocytes and hematopoietic cells

Andy Yu, Ningjie Hu, Mario Ostrowski

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

Poxviruses including canarypox (ALVAC) and vaccinia viruses have, in recent years, received considerable attention as live vectors for the development of vaccines against infectious diseases such as AIDS, malaria, and tuberculosis. However, the cellular targets for viral infection within the human immune system and the consequences of infection for cells involved in the generation of immune responses have not been clearly delineated. Using recombinant enhanced green fluorescence protein (EGFP)-expressing ALVAC and vaccinia viruses, we have focused here on a side-by-side comparison of ALVAC and vaccinia virus tropism for cells from human peripheral blood and bone marrow. Both ALVAC and vaccinia viruses showed a strong bias toward monocyte infection. ALVAC minimally infected CD19+ B cells and was unable to infect ex vivo NK cells and T lymphocytes, whereas vaccinia virus could infect B lymphocytes and NK cell populations. Vaccinia virus was also able to infect T lymphocytes at low but detectable levels which could be enhanced upon their activation. Both ALVAC and vaccinia viruses could infect immature monocyte-derived dendritic cells (MDDCs), but only ALVAC infection induced their subsequent maturation. Infection in human bone marrow cells showed that ALVAC infection was restricted to a myelomonocytoid cell-specific CD33+ cell population, while vaccinia virus showed a strong, but not exclusive, preference for these cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages309-328
Number of pages20
Volume515
ISBN (Print)9781934115879
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume515
ISSN (Print)10643745

Fingerprint

Poxviridae
Tropism
Vaccinia virus
Leukocytes
Infection
Natural Killer Cells
Canarypox virus
Monocytes
B-Lymphocytes
T-Lymphocytes
Virus Diseases
Bone Marrow Cells
Dendritic Cells
Population
Malaria
Communicable Diseases
Immune System
Acquired Immunodeficiency Syndrome
Tuberculosis
Vaccines

Keywords

  • ALVAC
  • Dendritic cell
  • Enhanced green fluorescence protein
  • Monocyte
  • Myeloid cell
  • Peripheral blood mononuclear cell
  • Vaccinia
  • Virus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yu, A., Hu, N., & Ostrowski, M. (2009). Poxvirus tropism for primary human leukocytes and hematopoietic cells. In Methods in Molecular Biology (Vol. 515, pp. 309-328). (Methods in Molecular Biology; Vol. 515). Humana Press Inc.. https://doi.org/10.1007/978-1-59745-559-6_22

Poxvirus tropism for primary human leukocytes and hematopoietic cells. / Yu, Andy; Hu, Ningjie; Ostrowski, Mario.

Methods in Molecular Biology. Vol. 515 Humana Press Inc., 2009. p. 309-328 (Methods in Molecular Biology; Vol. 515).

Research output: Chapter in Book/Report/Conference proceedingChapter

Yu, A, Hu, N & Ostrowski, M 2009, Poxvirus tropism for primary human leukocytes and hematopoietic cells. in Methods in Molecular Biology. vol. 515, Methods in Molecular Biology, vol. 515, Humana Press Inc., pp. 309-328. https://doi.org/10.1007/978-1-59745-559-6_22
Yu A, Hu N, Ostrowski M. Poxvirus tropism for primary human leukocytes and hematopoietic cells. In Methods in Molecular Biology. Vol. 515. Humana Press Inc. 2009. p. 309-328. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-559-6_22
Yu, Andy ; Hu, Ningjie ; Ostrowski, Mario. / Poxvirus tropism for primary human leukocytes and hematopoietic cells. Methods in Molecular Biology. Vol. 515 Humana Press Inc., 2009. pp. 309-328 (Methods in Molecular Biology).
@inbook{1bf50b8f7bfa47988f643997b7c53aa6,
title = "Poxvirus tropism for primary human leukocytes and hematopoietic cells",
abstract = "Poxviruses including canarypox (ALVAC) and vaccinia viruses have, in recent years, received considerable attention as live vectors for the development of vaccines against infectious diseases such as AIDS, malaria, and tuberculosis. However, the cellular targets for viral infection within the human immune system and the consequences of infection for cells involved in the generation of immune responses have not been clearly delineated. Using recombinant enhanced green fluorescence protein (EGFP)-expressing ALVAC and vaccinia viruses, we have focused here on a side-by-side comparison of ALVAC and vaccinia virus tropism for cells from human peripheral blood and bone marrow. Both ALVAC and vaccinia viruses showed a strong bias toward monocyte infection. ALVAC minimally infected CD19+ B cells and was unable to infect ex vivo NK cells and T lymphocytes, whereas vaccinia virus could infect B lymphocytes and NK cell populations. Vaccinia virus was also able to infect T lymphocytes at low but detectable levels which could be enhanced upon their activation. Both ALVAC and vaccinia viruses could infect immature monocyte-derived dendritic cells (MDDCs), but only ALVAC infection induced their subsequent maturation. Infection in human bone marrow cells showed that ALVAC infection was restricted to a myelomonocytoid cell-specific CD33+ cell population, while vaccinia virus showed a strong, but not exclusive, preference for these cells.",
keywords = "ALVAC, Dendritic cell, Enhanced green fluorescence protein, Monocyte, Myeloid cell, Peripheral blood mononuclear cell, Vaccinia, Virus",
author = "Andy Yu and Ningjie Hu and Mario Ostrowski",
year = "2009",
doi = "10.1007/978-1-59745-559-6_22",
language = "English (US)",
isbn = "9781934115879",
volume = "515",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "309--328",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Poxvirus tropism for primary human leukocytes and hematopoietic cells

AU - Yu, Andy

AU - Hu, Ningjie

AU - Ostrowski, Mario

PY - 2009

Y1 - 2009

N2 - Poxviruses including canarypox (ALVAC) and vaccinia viruses have, in recent years, received considerable attention as live vectors for the development of vaccines against infectious diseases such as AIDS, malaria, and tuberculosis. However, the cellular targets for viral infection within the human immune system and the consequences of infection for cells involved in the generation of immune responses have not been clearly delineated. Using recombinant enhanced green fluorescence protein (EGFP)-expressing ALVAC and vaccinia viruses, we have focused here on a side-by-side comparison of ALVAC and vaccinia virus tropism for cells from human peripheral blood and bone marrow. Both ALVAC and vaccinia viruses showed a strong bias toward monocyte infection. ALVAC minimally infected CD19+ B cells and was unable to infect ex vivo NK cells and T lymphocytes, whereas vaccinia virus could infect B lymphocytes and NK cell populations. Vaccinia virus was also able to infect T lymphocytes at low but detectable levels which could be enhanced upon their activation. Both ALVAC and vaccinia viruses could infect immature monocyte-derived dendritic cells (MDDCs), but only ALVAC infection induced their subsequent maturation. Infection in human bone marrow cells showed that ALVAC infection was restricted to a myelomonocytoid cell-specific CD33+ cell population, while vaccinia virus showed a strong, but not exclusive, preference for these cells.

AB - Poxviruses including canarypox (ALVAC) and vaccinia viruses have, in recent years, received considerable attention as live vectors for the development of vaccines against infectious diseases such as AIDS, malaria, and tuberculosis. However, the cellular targets for viral infection within the human immune system and the consequences of infection for cells involved in the generation of immune responses have not been clearly delineated. Using recombinant enhanced green fluorescence protein (EGFP)-expressing ALVAC and vaccinia viruses, we have focused here on a side-by-side comparison of ALVAC and vaccinia virus tropism for cells from human peripheral blood and bone marrow. Both ALVAC and vaccinia viruses showed a strong bias toward monocyte infection. ALVAC minimally infected CD19+ B cells and was unable to infect ex vivo NK cells and T lymphocytes, whereas vaccinia virus could infect B lymphocytes and NK cell populations. Vaccinia virus was also able to infect T lymphocytes at low but detectable levels which could be enhanced upon their activation. Both ALVAC and vaccinia viruses could infect immature monocyte-derived dendritic cells (MDDCs), but only ALVAC infection induced their subsequent maturation. Infection in human bone marrow cells showed that ALVAC infection was restricted to a myelomonocytoid cell-specific CD33+ cell population, while vaccinia virus showed a strong, but not exclusive, preference for these cells.

KW - ALVAC

KW - Dendritic cell

KW - Enhanced green fluorescence protein

KW - Monocyte

KW - Myeloid cell

KW - Peripheral blood mononuclear cell

KW - Vaccinia

KW - Virus

UR - http://www.scopus.com/inward/record.url?scp=65649113639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65649113639&partnerID=8YFLogxK

U2 - 10.1007/978-1-59745-559-6_22

DO - 10.1007/978-1-59745-559-6_22

M3 - Chapter

C2 - 19378122

AN - SCOPUS:65649113639

SN - 9781934115879

VL - 515

T3 - Methods in Molecular Biology

SP - 309

EP - 328

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -