POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1

Dong Kee Lee, Dongchul Suh, Howard Edenberg, Man Wook Hur

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The POZ domain is a protein-protein interaction motif that is found in many transcription factors, which are important for development, oncogenesis, apoptosis, and transcription repression. We cloned the POZ domain transcription factor, FBI-1, that recognizes the cis-element (bp -38 to -22) located just upstream of the core Sp1 binding sites (bp -22 to +22) of the ADH5/FDH minimal promoter (bp -38 to +61) in vitro and in vivo, as revealed by electrophoretic mobility shift assay and chromatin immunoprecipitation assay. The ADH5/FDH minimal promoter is potently repressed by the FBI-1. Glutathione S-transferase fusion protein pull-down showed that the POZ domains of FBI-1, Plzf, and Bcl-6 directly interact with the zinc finger DNA binding domain of Sp1. DNase I footprinting assays showed that the interaction prevents binding of Sp1 to the GC boxes of the ADH5/FDH promoter. Gal4-POZ domain fusions targeted proximal to the GC boxes repress transcription of the Gal4 upstream activator sequence-Sp1-adenovirus major late promoter. Our data suggest that POZ domain represses transcription by interacting with Sp1 zinc fingers and by interfering with the DNA binding activity of Sp1.

Original languageEnglish
Pages (from-to)26761-26768
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number30
DOIs
StatePublished - Jul 26 2002

Fingerprint

Zinc Fingers
Transcription
Zinc
Assays
Transcription Factors
DNA
Fusion reactions
Protein Interaction Domains and Motifs
Electrophoretic mobility
Deoxyribonuclease I
Glutathione Transferase
Chromatin
Proteins
Binding Sites
Apoptosis
Chromatin Immunoprecipitation
Electrophoretic Mobility Shift Assay
Adenoviridae
Carcinogenesis
BTB-POZ Domain

ASJC Scopus subject areas

  • Biochemistry

Cite this

POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1. / Lee, Dong Kee; Suh, Dongchul; Edenberg, Howard; Hur, Man Wook.

In: Journal of Biological Chemistry, Vol. 277, No. 30, 26.07.2002, p. 26761-26768.

Research output: Contribution to journalArticle

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