Two polyclonal activators of bacterial origin, peptidoglycan (PG) and lipopolysaccharide (LPS), preferentially induce high proportions of cells secreting rheumatoid factor and anti-single stranded (ss) DNA antibodies in mouse lymphocyte cultures. The in vivo polyclonal activation and induction of autoantibodies in PG- and LPS-injected mice were studied using the hemolytic plaque assay and its modifications with protein A-, mouse IgG-, or ssDNA-sensitized erythrocytes. The majority (ca. 60%) of immunoglobulin-secreting cells in normal uninjected or PG- or LPS-injected mice secreted rheumatoid factor antibodies. Less than 5% of these cells secreted anti-ssDNA antibodies, whereas the proportion of cells producing antibodies to bromelin-treated mouse red cells was 0.1%, on the average. In most animals, cell secreting antibodies to intact mouse red cells were not detected. An injection of PG or LPS caused a significant increase in the number of cells secreting most of these antibodies. This in vivo polyclonal activation was similar, in terms of the kinetics and specificities of antibodies produced, to the in vitro polyclonal activation. Its magnitude was, however, almost 10 times lower than the in vitro response. This difference could not be overcome by multiple injections of PG or LPS. These multiple injections induced, however, high numbers of cells secreting anti-ssDNA antibodies. Similar results were obtained in BALB/c, CBA/H, and C57BL/6 mice. Since these mouse strains have different H-2 types, there was no association of a single H-2 type with the ability to develop high numbers of polyclonal autoantibody secreting cells in vivo. Our results demonstrate that, like the in vitro system, in vivo-induced polyclonal activation results in a preferential stimulation of rheumatoid factor- and anti-ssDNA antibody-secreting cells. In normal mice, however, this stimulation is quite transient and less pronounced than in vitro.
|Original language||English (US)|
|Number of pages||5|
|Journal||Journal of Immunology|
|State||Published - Jan 1 1982|
ASJC Scopus subject areas
- Immunology and Allergy