Pregabalin and gabapentin reduce release of substance P and CGRP from rat spinal tissues only after inflammation or activation of protein kinase C

Jill Fehrenbacher, Charles P. Taylor, Michael Vasko

Research output: Contribution to journalArticle

259 Citations (Scopus)

Abstract

Gabapentin and pregabalin are amino acid derivatives of γ-amino butyric acid that have anticonvulsant, analgesic, and anxiolytic-like properties in animal models. The mechanisms of these effects, however, are not well understood. To ascertain whether these drugs have effects on sensory neurons, we studied their actions on capsaicin-evoked release of the sensory neuropeptides, substance P and calcitonin gene-related peptide from rat spinal cord slices in vitro. Although release of immunoreactive peptides from non-inflamed animals was not altered by either drug, prior in vivo treatment by intraplantar injection of complete Freund's adjuvant enhanced release from spinal tissues in vitro, which was attenuated by gabapentin and pregabalin. These drugs also reduced release of immunoreactive neuropeptides in spinal tissues pretreated in vitro with the protein kinase C activator, phorbol 12,13-dibutyrate. Our results suggest that gabapentin and pregabalin modulate the release of sensory neuropeptides, but only under conditions corresponding to significant inflammation-induced sensitization of the spinal cord.

Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalPain
Volume105
Issue number1-2
DOIs
StatePublished - Sep 2003

Fingerprint

Substance P
Neuropeptides
Protein Kinase C
Inflammation
Spinal Cord
Pharmaceutical Preparations
Phorbol 12,13-Dibutyrate
Butyric Acid
Freund's Adjuvant
Calcitonin Gene-Related Peptide
Capsaicin
Anti-Anxiety Agents
Sensory Receptor Cells
Anticonvulsants
Analgesics
Animal Models
Amino Acids
Peptides
Injections
Pregabalin

Keywords

  • Gabapentin
  • Inflammation
  • Neuropeptide release
  • Protein kinase C

ASJC Scopus subject areas

  • Clinical Neurology
  • Psychiatry and Mental health
  • Neurology
  • Neuroscience(all)
  • Pharmacology
  • Clinical Psychology

Cite this

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