Pregenomic RNA is required for efficient incorporation of Pol polyprotein into foamy virus capsids

Martin Heinkelein, Cordula Leurs, Matthias Rammling, Katrin Peters, Helmut Hanenberg, Axel Rethwilm

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The foamy virus (FV) Pol polyprotein is translated independently of Gag from a spliced mRNA. This method of expression raises the question of how Pol is associated with the viral particle. Using a transient FV vector transfection system, it is shown that pregenomic RNA is required for efficient virion incorporation of functionally active Pol and that protein-protein interactions of Pol with Gag are not sufficient to complete particle assembly.

Original languageEnglish (US)
Pages (from-to)10069-10073
Number of pages5
JournalJournal of Virology
Volume76
Issue number19
DOIs
StatePublished - Oct 2002
Externally publishedYes

Fingerprint

pol Gene Products
Spumavirus
capsid
Capsid
virion
RNA
Virion
viruses
protein-protein interactions
transfection
Transfection
Messenger RNA
polyproteins
methodology

ASJC Scopus subject areas

  • Immunology

Cite this

Heinkelein, M., Leurs, C., Rammling, M., Peters, K., Hanenberg, H., & Rethwilm, A. (2002). Pregenomic RNA is required for efficient incorporation of Pol polyprotein into foamy virus capsids. Journal of Virology, 76(19), 10069-10073. https://doi.org/10.1128/JVI.76.19.10069-10073.2002

Pregenomic RNA is required for efficient incorporation of Pol polyprotein into foamy virus capsids. / Heinkelein, Martin; Leurs, Cordula; Rammling, Matthias; Peters, Katrin; Hanenberg, Helmut; Rethwilm, Axel.

In: Journal of Virology, Vol. 76, No. 19, 10.2002, p. 10069-10073.

Research output: Contribution to journalArticle

Heinkelein, M, Leurs, C, Rammling, M, Peters, K, Hanenberg, H & Rethwilm, A 2002, 'Pregenomic RNA is required for efficient incorporation of Pol polyprotein into foamy virus capsids', Journal of Virology, vol. 76, no. 19, pp. 10069-10073. https://doi.org/10.1128/JVI.76.19.10069-10073.2002
Heinkelein, Martin ; Leurs, Cordula ; Rammling, Matthias ; Peters, Katrin ; Hanenberg, Helmut ; Rethwilm, Axel. / Pregenomic RNA is required for efficient incorporation of Pol polyprotein into foamy virus capsids. In: Journal of Virology. 2002 ; Vol. 76, No. 19. pp. 10069-10073.
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