Preirradiation of host cells does not alter blockage of simian virus 40 replication forks by pyrimidine dimers

Abraham Scaria, Howard J. Edenberg

Research output: Contribution to journalArticle

4 Scopus citations


Do damage-inducible responses in mammalian cells alter the interaction of lesions with replication forks? We have previously demonstrated that preirradiation of the host cell mitigates UV inhibition of SV40 DNA replication; this mitigation can be detected within the first 30 min after the test irradiation. Here we test the hypothesis that this mitigation involves either (1) rapid dimer removal, (2) rapid synthesis of daughter strands past lesions (trans-dimer synthesis), or (3) continued progression of the replication fork beyond a dimer. Cells preirradiated with UV were infected with undamaged SV40, and the effects of UV upon viral DNA synthesis were measured within the first hour after a subsequent test iirradiation. In preirradiated cells, as well as in non-preirradiated cells, pyrimidine dimers block elongation of daughter strands; daughter strands grow only to a size equal to the interdimer distance along the parental strands. There is, within this first hour after UV, no evidence for trans-dimer synthesis, nor for more rapid dimer removal either in the bulk of the parental DNA or in molecules in the replication pool. Progression of the replication forks was analyzed by electron microscopy or replicating SV40 molecules. Dimers block replication-fork progression in preirradiated cells to the same extent as in non-preirradiated cells. These experiments argue strongly against the hypothesis that preirradiation of host cells results in either the rapid removal of dimers, trans-dimer synthesis, or continued replication-fork progression beyond dimers.

Original languageEnglish (US)
Pages (from-to)11-20
Number of pages10
JournalMutation Research DNA Repair Reports
Issue number1
StatePublished - Jan 1988


  • DNA replication
  • Inducible phenomena
  • Inhibition of replication
  • Preirradiation
  • Pyrimidine dimers
  • Replication forks
  • SV40
  • Ultraviolet radiation

ASJC Scopus subject areas

  • Medicine(all)

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