Preirradiation of host (monkey) cells mitigates the effects of UV upon simian virus 40 DNA replication

A. Scaria, Howard Edenberg

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We are examining the effects of preirradiation of host (monkey) cells upon the replication of UV-damaged SV40. Control cells and cells preirradiated with low fluences (5 or 10 J/m2) of UV were infected with undamaged SV40, and the immediate effects of a subsequent irradiation were determined. UV inhibited total SV 40 DNA synthesis (incorporation of thymidine into viral DNA) in both preirradiated and control cells, but the extent of inhibition was less in the preirradiated cells. A test fluence of 60 J/m2 to SW40 replicating in preirradiated cells reduced synthesis only as much as a test fluence of 25 J/m2 in control cells. The fraction of recently replicated SV40 molecules that re-entered the replication pool and subsequently completed one round of replication in the first 2 h after UV was also decreased less in the prerradiated cells. Thus preirradiation of the host cell mitigates the immediate inhibitory effects of a subsequent UV exposure upon SV40 replication.

Original languageEnglish
Pages (from-to)265-271
Number of pages7
JournalMutation Research DNA Repair Reports
Volume183
Issue number3
DOIs
StatePublished - 1987

Fingerprint

Simian virus 40
DNA Replication
Haplorhini
Viral DNA
Thymidine
DNA

Keywords

  • (Ultraviolet light)
  • DNA repair
  • DNA replication
  • Enhanced survival
  • Pre-irradation
  • Primate cells
  • Simian virus 40
  • SOS response

Cite this

Preirradiation of host (monkey) cells mitigates the effects of UV upon simian virus 40 DNA replication. / Scaria, A.; Edenberg, Howard.

In: Mutation Research DNA Repair Reports, Vol. 183, No. 3, 1987, p. 265-271.

Research output: Contribution to journalArticle

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