Preliminary evaluation of a load-bearing BMP-2 carrier for segmental defect regeneration

T.M. Gabriel Chu, Peter Sargent, Stuart J. Warden, Charles H. Turner, Rena L. Stewart

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Large segmental defects in bones can result from tumor removal, massive trauma, congenital malformation, or non-union fractures. Such defects often are difficult to manage and require multiple-phase surgery to achieve adequate union and function. In this study, we propose a novel design of bone morphogenetic protein 2 (BMP-2) carrier for tissue engineering of segmental defect regeneration. The tube-shaped BMP-2 carrier was fabrication from a poly(propylene fumarate)/tricalcium phosphate (PPF/TCP) composite via casting technique developed in our laboratory. An in vitro evaluation showed that the compressive strength of the carrier decreased about 48% in 12 weeks while maintained a pH in the 6.8-7.4 range. In vivo study was conducted by implanting carriers loaded with 10 μg of BMP-2 in 5 mm rat femur gap model for 15 weeks. X-ray evidence of bridging was first found in the BMP group at 3 weeks. Bridging in all animals (N=4) in the BMP group was found at 9 weeks. No x-ray evidence of bridging was found in the No BMP group (N=3). pQCT analysis indicated that the bone mineral density of the callus in the BMP group has reached the level of native femur at 15 weeks after implantation, while the callus in the No BMP group has a bone mineral density at a lower level of 84% to the native femur. Histology analysis shows that a normal fatty bone marrow was restored and mineralized callus formed and bridged the segmental defect.

Original languageEnglish
Pages (from-to)42-47
Number of pages6
JournalBiomedical Sciences Instrumentation
Volume42
StatePublished - Apr 2006

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Bearings (structural)
Bone Morphogenetic Protein 2
Bony Callus
Weight-Bearing
Femur
Regeneration
Bone
Proteins
Bone Density
Defects
X-Rays
Compressive Strength
Tissue Engineering
Histology
Bone Marrow
Minerals
Bone and Bones
Wounds and Injuries
X rays
Tissue engineering

Keywords

  • BMP-2
  • Bone mineral density
  • Poly(propylene) fumarate
  • Rapid prototyping
  • Scaffold
  • Segmental defect

ASJC Scopus subject areas

  • Hardware and Architecture

Cite this

Preliminary evaluation of a load-bearing BMP-2 carrier for segmental defect regeneration. / Chu, T.M. Gabriel; Sargent, Peter; Warden, Stuart J.; Turner, Charles H.; Stewart, Rena L.

In: Biomedical Sciences Instrumentation, Vol. 42, 04.2006, p. 42-47.

Research output: Contribution to journalArticle

Chu, T.M. Gabriel ; Sargent, Peter ; Warden, Stuart J. ; Turner, Charles H. ; Stewart, Rena L. / Preliminary evaluation of a load-bearing BMP-2 carrier for segmental defect regeneration. In: Biomedical Sciences Instrumentation. 2006 ; Vol. 42. pp. 42-47.
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