Prenatal identification of potential donors for umbilical cord blood transplantation for Fanconi anemia

A. D. Auerbach, Q. Liu, R. Ghosh, M. S. Pollack, Hal Broxmeyer, H. E. Broxmeyer

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Reported here are studies of Fanconi anemia fetal cells that led to the first use of umbilical cord blood for hematopoietic reconstitution in a clinical trial. Prenatal diagnosis and HLA typing were performed in fetuses at risk for Fanconi anemia (FA) to identify, prior to birth, those that were unaffected with the syndrome and were HLA-identical to affected siblings. Umbilical cord blood was harvested at the delivery of these infants; assays of progenitor cells indicated the presence of colony-forming units-granulocyte-macrophage (CFU-GM) in numbers similar to those of bone marrow CFU-GM that are associated with successful engraftment in HLA-matched allogeneic bone marrow transplantation. The possibility that umbilical cord blood from a single individual can be used as an alternative to bone marrow for hematopoietic reconstitution has now been demonstrated by the successful engraftment of two patients with FA. Progenitor cell assays of umbilical cord blood collected at the birth of a child affected with FA, who had been misdiagnosed on the basis of chorionic villus sampling (CVS) studies, indicated a profound deficiency in colony formation, consistent with previously reported abnormalities in the growth of FA cells in vitro. These results suggest that the hematopoietic disorder in FA is related to an underlying problem with cell proliferation.

Original languageEnglish (US)
Pages (from-to)682-687
Number of pages6
JournalTransfusion
Volume30
Issue number8
StatePublished - 1990
Externally publishedYes

Fingerprint

Fanconi Anemia
Fetal Blood
Transplantation
Tissue Donors
Granulocyte-Macrophage Progenitor Cells
Stem Cells
Bone Marrow
Parturition
Chorionic Villi Sampling
Histocompatibility Testing
Sampling Studies
Homologous Transplantation
Diagnostic Errors
Prenatal Diagnosis
Bone Marrow Transplantation
Siblings
Fetus
Cell Proliferation
Clinical Trials
Growth

ASJC Scopus subject areas

  • Immunology
  • Hematology

Cite this

Auerbach, A. D., Liu, Q., Ghosh, R., Pollack, M. S., Broxmeyer, H., & Broxmeyer, H. E. (1990). Prenatal identification of potential donors for umbilical cord blood transplantation for Fanconi anemia. Transfusion, 30(8), 682-687.

Prenatal identification of potential donors for umbilical cord blood transplantation for Fanconi anemia. / Auerbach, A. D.; Liu, Q.; Ghosh, R.; Pollack, M. S.; Broxmeyer, Hal; Broxmeyer, H. E.

In: Transfusion, Vol. 30, No. 8, 1990, p. 682-687.

Research output: Contribution to journalArticle

Auerbach, AD, Liu, Q, Ghosh, R, Pollack, MS, Broxmeyer, H & Broxmeyer, HE 1990, 'Prenatal identification of potential donors for umbilical cord blood transplantation for Fanconi anemia', Transfusion, vol. 30, no. 8, pp. 682-687.
Auerbach, A. D. ; Liu, Q. ; Ghosh, R. ; Pollack, M. S. ; Broxmeyer, Hal ; Broxmeyer, H. E. / Prenatal identification of potential donors for umbilical cord blood transplantation for Fanconi anemia. In: Transfusion. 1990 ; Vol. 30, No. 8. pp. 682-687.
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