Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887

Lawrence P. Garetto, Mary R. Gonsalves, Emily R. Morey, G. Durnova, W. Eugene Roberts

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The influence of 12.5 days of spaceflight and a 55 h stressful recovery period (at 1 g) on fibroblastlike osteoblast precursor cells was assessed in the periodontal ligament (PDL) of rats that were 91 days old at launch. Nuclear morphometry was used as a marker for precursor cell differentiation in 3 μm sections cut in the midsagittal plane from the maxillary first molar. According to nuclear volume, cells were classified as pre-osteoblasts (C + D cells, ≥120 μm3) and less differentiated progenitor cells (A + A' cells, 40-79 μm3). Compared with synchronous controls (simulated flight conditions), the 55 h postflight recovery period at 1 g resulted in a 40% decrease in the A + A' cell population, a 42% increase in the C + D cells, and a 39% increase in the number of PDL fibroblastlike cells near the bone surface. These results are consistent with a postflight osteogenic response in PDL. This recovery response occurred despite physiological stress in the flight animals that resulted in a highly significant (P ≤ 0.001) increase in adrenal weight. The data suggest that after spaceflight there is a strong and rapid recovery mechanism for osteoblast differentiation that is not suppressed by physiological stress.

Original languageEnglish (US)
Pages (from-to)24-28
Number of pages5
JournalFASEB Journal
Volume4
Issue number1
StatePublished - 1990
Externally publishedYes

Fingerprint

Cosmos
space flight
Space Flight
Periodontal Ligament
Ligaments
Osteoblasts
Recovery
Physiological Stress
Somatostatin-Secreting Cells
osteoblasts
ligaments
Animal Flight
Cell Nucleus Size
cells
flight
Rats
Cell Differentiation
Bone
Animals
Stem Cells

Keywords

  • Bone
  • Cell differentiation
  • Microgravity
  • Osteogenesis
  • Physiological stress

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Garetto, L. P., Gonsalves, M. R., Morey, E. R., Durnova, G., & Eugene Roberts, W. (1990). Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887. FASEB Journal, 4(1), 24-28.

Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887. / Garetto, Lawrence P.; Gonsalves, Mary R.; Morey, Emily R.; Durnova, G.; Eugene Roberts, W.

In: FASEB Journal, Vol. 4, No. 1, 1990, p. 24-28.

Research output: Contribution to journalArticle

Garetto, LP, Gonsalves, MR, Morey, ER, Durnova, G & Eugene Roberts, W 1990, 'Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887', FASEB Journal, vol. 4, no. 1, pp. 24-28.
Garetto LP, Gonsalves MR, Morey ER, Durnova G, Eugene Roberts W. Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887. FASEB Journal. 1990;4(1):24-28.
Garetto, Lawrence P. ; Gonsalves, Mary R. ; Morey, Emily R. ; Durnova, G. ; Eugene Roberts, W. / Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887. In: FASEB Journal. 1990 ; Vol. 4, No. 1. pp. 24-28.
@article{ddfc5efb459e42bea25945be1f1eb7ff,
title = "Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887",
abstract = "The influence of 12.5 days of spaceflight and a 55 h stressful recovery period (at 1 g) on fibroblastlike osteoblast precursor cells was assessed in the periodontal ligament (PDL) of rats that were 91 days old at launch. Nuclear morphometry was used as a marker for precursor cell differentiation in 3 μm sections cut in the midsagittal plane from the maxillary first molar. According to nuclear volume, cells were classified as pre-osteoblasts (C + D cells, ≥120 μm3) and less differentiated progenitor cells (A + A' cells, 40-79 μm3). Compared with synchronous controls (simulated flight conditions), the 55 h postflight recovery period at 1 g resulted in a 40{\%} decrease in the A + A' cell population, a 42{\%} increase in the C + D cells, and a 39{\%} increase in the number of PDL fibroblastlike cells near the bone surface. These results are consistent with a postflight osteogenic response in PDL. This recovery response occurred despite physiological stress in the flight animals that resulted in a highly significant (P ≤ 0.001) increase in adrenal weight. The data suggest that after spaceflight there is a strong and rapid recovery mechanism for osteoblast differentiation that is not suppressed by physiological stress.",
keywords = "Bone, Cell differentiation, Microgravity, Osteogenesis, Physiological stress",
author = "Garetto, {Lawrence P.} and Gonsalves, {Mary R.} and Morey, {Emily R.} and G. Durnova and {Eugene Roberts}, W.",
year = "1990",
language = "English (US)",
volume = "4",
pages = "24--28",
journal = "FASEB Journal",
issn = "0892-6638",
publisher = "FASEB",
number = "1",

}

TY - JOUR

T1 - Preosteoblast production 55 hours after a 12.5-day spaceflight on Cosmos 1887

AU - Garetto, Lawrence P.

AU - Gonsalves, Mary R.

AU - Morey, Emily R.

AU - Durnova, G.

AU - Eugene Roberts, W.

PY - 1990

Y1 - 1990

N2 - The influence of 12.5 days of spaceflight and a 55 h stressful recovery period (at 1 g) on fibroblastlike osteoblast precursor cells was assessed in the periodontal ligament (PDL) of rats that were 91 days old at launch. Nuclear morphometry was used as a marker for precursor cell differentiation in 3 μm sections cut in the midsagittal plane from the maxillary first molar. According to nuclear volume, cells were classified as pre-osteoblasts (C + D cells, ≥120 μm3) and less differentiated progenitor cells (A + A' cells, 40-79 μm3). Compared with synchronous controls (simulated flight conditions), the 55 h postflight recovery period at 1 g resulted in a 40% decrease in the A + A' cell population, a 42% increase in the C + D cells, and a 39% increase in the number of PDL fibroblastlike cells near the bone surface. These results are consistent with a postflight osteogenic response in PDL. This recovery response occurred despite physiological stress in the flight animals that resulted in a highly significant (P ≤ 0.001) increase in adrenal weight. The data suggest that after spaceflight there is a strong and rapid recovery mechanism for osteoblast differentiation that is not suppressed by physiological stress.

AB - The influence of 12.5 days of spaceflight and a 55 h stressful recovery period (at 1 g) on fibroblastlike osteoblast precursor cells was assessed in the periodontal ligament (PDL) of rats that were 91 days old at launch. Nuclear morphometry was used as a marker for precursor cell differentiation in 3 μm sections cut in the midsagittal plane from the maxillary first molar. According to nuclear volume, cells were classified as pre-osteoblasts (C + D cells, ≥120 μm3) and less differentiated progenitor cells (A + A' cells, 40-79 μm3). Compared with synchronous controls (simulated flight conditions), the 55 h postflight recovery period at 1 g resulted in a 40% decrease in the A + A' cell population, a 42% increase in the C + D cells, and a 39% increase in the number of PDL fibroblastlike cells near the bone surface. These results are consistent with a postflight osteogenic response in PDL. This recovery response occurred despite physiological stress in the flight animals that resulted in a highly significant (P ≤ 0.001) increase in adrenal weight. The data suggest that after spaceflight there is a strong and rapid recovery mechanism for osteoblast differentiation that is not suppressed by physiological stress.

KW - Bone

KW - Cell differentiation

KW - Microgravity

KW - Osteogenesis

KW - Physiological stress

UR - http://www.scopus.com/inward/record.url?scp=0025191392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025191392&partnerID=8YFLogxK

M3 - Article

VL - 4

SP - 24

EP - 28

JO - FASEB Journal

JF - FASEB Journal

SN - 0892-6638

IS - 1

ER -