Preparation and crystallization of human transthyretin (prealbumin) variants

L. K. Steinrauf, Youjia Cao, Jean Hamilton, Jill Murrell, Juris J. Liepnieks, Merrill D. Benson

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Naturally occurring variants of human serum transthyretin (prealbumin) have been prepared by recombinant DNA methods and crystallized from ammonium sulfate solutions to give crystals suitable for x-ray crystallographic analysis. Included are variants which are known to be associated with familial amyloidotic polyneuropathy. Dyes which have been used as histochemical stains to identify amyloid tissue deposits: Congo Red, Methylene Blue and Bromophenol Blue, have been co-crystallized with the transthyretin variants. Congo Red was found to be very selective while Methylene Blue actually assisted in the formation of crystals. All crystal forms which were examined were isomorphous to the structure of normal transthyretin.

Original languageEnglish (US)
Pages (from-to)804-809
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume179
Issue number2
DOIs
StatePublished - Sep 16 1991

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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