Preparation of rat intestinal muscle and mucosa for quantitative microcirculatory studies

H. Glenn Bohlen, Robert W. Gore

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

This paper describes methods and procedural details for preparing either the rat intestinal muscle or mucosa for observation under the microscope. The tissues are transilluminated through a short optical clad rod with a standard condenser system. The tissue environment can be controlled, and the microcirculation of the muscle and mucosa are clearly visible and directly accessible. The arteries, veins, and nerves to the intestine can be simultaneously manipulated. One can therefore control a number of macro- and microcirculatory parameters, and perform a wide variety of quantitative measurements on either the intestinal muscle or mucosal vasculature.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalMicrovascular Research
Volume11
Issue number1
DOIs
StatePublished - Jan 1976
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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