Preparation of viable tumour cell vaccine from human solid tumours: Relationship between tumour mass and cell yield

Theodore Logan, W. Shannon, J. Bryant, P. Kane, N. Wolmark, M. Posner, J. M. Kirkwood, M. S. Ernstoff, J. W. Futrell, L. D. Straw, S. Iwatuski, R. Bahnson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Active specific Immunotherapy for cancer often requires the use of autologous or allogeneic tumour cells as Immunizing antigen. Tumours were obtained for such a protocol. However, estimation of viable cell yield from pre-processed fresh tumour mass was difficult, and Initially there did not appear to be a direct relationship between pre-processed tumour mass and viable cells obtained after processing. We therefore analysed all of 293 tumour specimens processed to attempt to discern such a relationship. Of these 137 were melanoma, 14 were sarcoma, 48 were adenocarcinoma, 59 were renal cell carcinoma and 35 were classified as other. A positive correlation was found between pre-processed tumour mass and viable cell yield, with Spearman correlation values varying from r = 0.49 (adenocarcinoma) to r = 0.84 (melanoma). For all tumours the Spearman correlation was r=0.70 (p = 0.0001). Not surprisingly, the most frequent site of removal associated with bacterial contamination was bowel. In conclusion, this study provides useful curves for predicting viable tumour cell yield from pre-processed tumour mass of given histology.

Original languageEnglish (US)
Pages (from-to)451-456
Number of pages6
JournalMelanoma Research
Volume3
Issue number6
StatePublished - 1993
Externally publishedYes

Fingerprint

Cancer Vaccines
Neoplasms
Melanoma
Adenocarcinoma
Active Immunotherapy
Renal Cell Carcinoma
Sarcoma
Histology
Antigens

Keywords

  • Active specific immunotherapy
  • Tumour cell vaccine
  • Tumour cell yield

ASJC Scopus subject areas

  • Cancer Research
  • Dermatology
  • Oncology

Cite this

Logan, T., Shannon, W., Bryant, J., Kane, P., Wolmark, N., Posner, M., ... Bahnson, R. (1993). Preparation of viable tumour cell vaccine from human solid tumours: Relationship between tumour mass and cell yield. Melanoma Research, 3(6), 451-456.

Preparation of viable tumour cell vaccine from human solid tumours : Relationship between tumour mass and cell yield. / Logan, Theodore; Shannon, W.; Bryant, J.; Kane, P.; Wolmark, N.; Posner, M.; Kirkwood, J. M.; Ernstoff, M. S.; Futrell, J. W.; Straw, L. D.; Iwatuski, S.; Bahnson, R.

In: Melanoma Research, Vol. 3, No. 6, 1993, p. 451-456.

Research output: Contribution to journalArticle

Logan, T, Shannon, W, Bryant, J, Kane, P, Wolmark, N, Posner, M, Kirkwood, JM, Ernstoff, MS, Futrell, JW, Straw, LD, Iwatuski, S & Bahnson, R 1993, 'Preparation of viable tumour cell vaccine from human solid tumours: Relationship between tumour mass and cell yield', Melanoma Research, vol. 3, no. 6, pp. 451-456.
Logan, Theodore ; Shannon, W. ; Bryant, J. ; Kane, P. ; Wolmark, N. ; Posner, M. ; Kirkwood, J. M. ; Ernstoff, M. S. ; Futrell, J. W. ; Straw, L. D. ; Iwatuski, S. ; Bahnson, R. / Preparation of viable tumour cell vaccine from human solid tumours : Relationship between tumour mass and cell yield. In: Melanoma Research. 1993 ; Vol. 3, No. 6. pp. 451-456.
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