Preparing undercut model of posttraumatic epileptogenesis in rodents.

Wenhui Xiong, Xingjie Ping, Jianhua Gao, Xiaoming Jin

Research output: Contribution to journalArticle

Abstract

Partially isolated cortex ("undercut") is an animal model of posttraumatic epileptogenesis. The surgical procedure involves cutting through the sensorimotor cortex and the underneath white matter (undercut) so that a specific region of the cerebral cortex is largely isolated from the neighboring cortex and subcortical regions(1-3). After a latency of two or more weeks following the surgery, epileptiform discharges can be recorded in brain slices from rodents(1); and electrical or behavior seizures can be observed in vivo from other species such as cat and monkey(4-6). This well established animal model is efficient to generate and mimics several important characteristics of traumatic brain injury. However, it is technically challenging attempting to make precise cortical lesions in the small rodent brain with a free hand. Based on the procedure initially established in Dr. David Prince's lab at the Stanford University(1), here we present an improved technique to perform a surgery for the preparation of this model in mice and rats. We demonstrate how to make a simple surgical device and use it to gain a better control of cutting depth and angle to generate more precise and consistent results. The device is easy to make, and the procedure is quick to learn. The generation of this animal model provides an efficient system for study on the mechanisms of posttraumatic epileptogenesis.

Original languageEnglish (US)
JournalJournal of visualized experiments : JoVE
Issue number55
StatePublished - 2011
Externally publishedYes

Fingerprint

Rodentia
Animal Models
Brain
Animals
Surgery
Equipment and Supplies
Cerebral Cortex
Haplorhini
Seizures
Cats
Hand
Rats
Sensorimotor Cortex
Traumatic Brain Injury
White Matter

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Preparing undercut model of posttraumatic epileptogenesis in rodents. / Xiong, Wenhui; Ping, Xingjie; Gao, Jianhua; Jin, Xiaoming.

In: Journal of visualized experiments : JoVE, No. 55, 2011.

Research output: Contribution to journalArticle

@article{b98b10eea425477292fcc08cb4041a6c,
title = "Preparing undercut model of posttraumatic epileptogenesis in rodents.",
abstract = "Partially isolated cortex ({"}undercut{"}) is an animal model of posttraumatic epileptogenesis. The surgical procedure involves cutting through the sensorimotor cortex and the underneath white matter (undercut) so that a specific region of the cerebral cortex is largely isolated from the neighboring cortex and subcortical regions(1-3). After a latency of two or more weeks following the surgery, epileptiform discharges can be recorded in brain slices from rodents(1); and electrical or behavior seizures can be observed in vivo from other species such as cat and monkey(4-6). This well established animal model is efficient to generate and mimics several important characteristics of traumatic brain injury. However, it is technically challenging attempting to make precise cortical lesions in the small rodent brain with a free hand. Based on the procedure initially established in Dr. David Prince's lab at the Stanford University(1), here we present an improved technique to perform a surgery for the preparation of this model in mice and rats. We demonstrate how to make a simple surgical device and use it to gain a better control of cutting depth and angle to generate more precise and consistent results. The device is easy to make, and the procedure is quick to learn. The generation of this animal model provides an efficient system for study on the mechanisms of posttraumatic epileptogenesis.",
author = "Wenhui Xiong and Xingjie Ping and Jianhua Gao and Xiaoming Jin",
year = "2011",
language = "English (US)",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "55",

}

TY - JOUR

T1 - Preparing undercut model of posttraumatic epileptogenesis in rodents.

AU - Xiong, Wenhui

AU - Ping, Xingjie

AU - Gao, Jianhua

AU - Jin, Xiaoming

PY - 2011

Y1 - 2011

N2 - Partially isolated cortex ("undercut") is an animal model of posttraumatic epileptogenesis. The surgical procedure involves cutting through the sensorimotor cortex and the underneath white matter (undercut) so that a specific region of the cerebral cortex is largely isolated from the neighboring cortex and subcortical regions(1-3). After a latency of two or more weeks following the surgery, epileptiform discharges can be recorded in brain slices from rodents(1); and electrical or behavior seizures can be observed in vivo from other species such as cat and monkey(4-6). This well established animal model is efficient to generate and mimics several important characteristics of traumatic brain injury. However, it is technically challenging attempting to make precise cortical lesions in the small rodent brain with a free hand. Based on the procedure initially established in Dr. David Prince's lab at the Stanford University(1), here we present an improved technique to perform a surgery for the preparation of this model in mice and rats. We demonstrate how to make a simple surgical device and use it to gain a better control of cutting depth and angle to generate more precise and consistent results. The device is easy to make, and the procedure is quick to learn. The generation of this animal model provides an efficient system for study on the mechanisms of posttraumatic epileptogenesis.

AB - Partially isolated cortex ("undercut") is an animal model of posttraumatic epileptogenesis. The surgical procedure involves cutting through the sensorimotor cortex and the underneath white matter (undercut) so that a specific region of the cerebral cortex is largely isolated from the neighboring cortex and subcortical regions(1-3). After a latency of two or more weeks following the surgery, epileptiform discharges can be recorded in brain slices from rodents(1); and electrical or behavior seizures can be observed in vivo from other species such as cat and monkey(4-6). This well established animal model is efficient to generate and mimics several important characteristics of traumatic brain injury. However, it is technically challenging attempting to make precise cortical lesions in the small rodent brain with a free hand. Based on the procedure initially established in Dr. David Prince's lab at the Stanford University(1), here we present an improved technique to perform a surgery for the preparation of this model in mice and rats. We demonstrate how to make a simple surgical device and use it to gain a better control of cutting depth and angle to generate more precise and consistent results. The device is easy to make, and the procedure is quick to learn. The generation of this animal model provides an efficient system for study on the mechanisms of posttraumatic epileptogenesis.

UR - http://www.scopus.com/inward/record.url?scp=84856381256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856381256&partnerID=8YFLogxK

M3 - Article

C2 - 21946525

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 55

ER -