Preponderance of free mediator in the yeast Saccharomyces cerevisiae

Yuichiro Takagi, James Z. Chadick, Joshua A. Davis, Francisco J. Asturias

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Biochemical evidence suggesting that the predominant form of Mediator in the yeast Saccharomyces cerevisiae might be one in which the complex is associated with RNA polymerase II to form a holoenzyme has led to the proposition of a holoenzyme-based model for transcription initiation. We report that polymerase-free Mediator, isolated early on during a whole-cell extract fractionation protocol, is in fact the most abundant form of the Mediator complex. The existence of free Mediator would make possible independent recruitment of Mediator and RNA polymerase II to the preinitiation complex. This is in agreement with reports from in vivo studies of time and spatial independence of Mediator and RNA polymerase II promoter interaction, with current models of preinitiation complex structure in which promoter DNA upstream of the transcription start site is positioned between Mediator and polymerase, and with the proposed role of Mediator as the major component of the Scaffold complex involved in transcription reinitiation.

Original languageEnglish (US)
Pages (from-to)31200-31207
Number of pages8
JournalJournal of Biological Chemistry
Volume280
Issue number35
DOIs
StatePublished - Sep 2 2005

Fingerprint

RNA Polymerase II
Yeast
Saccharomyces cerevisiae
Holoenzymes
Yeasts
Transcription
Mediator Complex
Cell Fractionation
Transcription Initiation Site
Fractionation
Cell Extracts
Scaffolds
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Preponderance of free mediator in the yeast Saccharomyces cerevisiae. / Takagi, Yuichiro; Chadick, James Z.; Davis, Joshua A.; Asturias, Francisco J.

In: Journal of Biological Chemistry, Vol. 280, No. 35, 02.09.2005, p. 31200-31207.

Research output: Contribution to journalArticle

Takagi, Yuichiro ; Chadick, James Z. ; Davis, Joshua A. ; Asturias, Francisco J. / Preponderance of free mediator in the yeast Saccharomyces cerevisiae. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 35. pp. 31200-31207.
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