Preprogrammed, parallel on-chip immunoassay using system-level capillarity control

Sung Jin Kim, Sophie Paczesny, Shuichi Takayama, Katsuo Kurabayashi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Fully manual use of conventional multiwell plates makes enzyme-linked immunosorbent assay (ELISA)-based immunoassays highly time-consuming and labor-intensive. Here, we present a capillarity-driven on-chip immunoassay that greatly saves time and labor with an inexpensive setup. Our immunoassay process starts with pipetting multiple solutions into multiwells constructed on a microfluidic device chip. Subsequently, capillarity spontaneously transports multiple sample solutions and common reagent solutions into assigned detection channels on the chip in a purely passive and preprogrammed manner. Our device implements capillarity-driven immunoassays involving four sample and six reagent solutions within 30 min by orchestrating the functions of on-chip passive components. Notably, our immunoassay technique reduces the total number of pipetting processes by ∼5 times, as compared to assays on multiwell plates (48 vs 10). This assay technique allows us to quantify the concentrations of C-reactive protein and suppressor of tumorigenicity 2 with a detection limit of 8 and 90 pM, respectively. This device should be useful for sophisticated, parallel biochemical microfluidic processing in point-of-care settings under limited resources.

Original languageEnglish
Pages (from-to)6902-6907
Number of pages6
JournalAnalytical Chemistry
Volume85
Issue number14
DOIs
StatePublished - Jul 16 2013

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Capillarity
Assays
Microfluidics
Personnel
Immunosorbents
C-Reactive Protein
Enzymes
Processing

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Preprogrammed, parallel on-chip immunoassay using system-level capillarity control. / Kim, Sung Jin; Paczesny, Sophie; Takayama, Shuichi; Kurabayashi, Katsuo.

In: Analytical Chemistry, Vol. 85, No. 14, 16.07.2013, p. 6902-6907.

Research output: Contribution to journalArticle

Kim, Sung Jin ; Paczesny, Sophie ; Takayama, Shuichi ; Kurabayashi, Katsuo. / Preprogrammed, parallel on-chip immunoassay using system-level capillarity control. In: Analytical Chemistry. 2013 ; Vol. 85, No. 14. pp. 6902-6907.
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