Presence of luteinizing hormone-releasing hormone (Lhrh) mrna in rat spleen lymphocytes

Nasrin Azad, Nicholas V. Emanuele, Margaret M. Halloran, John Tentler, Mark R. Kelley

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Pursuant to our report of an immunoreactive and bioactive luteinizing hormone-releasing hormone (LHRH)-like molecule in rat spleen lymphocytes, we sought to determine whether these cells were capable of synthesizing LHRH by determining whether lymphocytes contain LHRH mRNA. To do this, total RNA was extracted from hypothalamic tissue, anterior pituitaries and from lymphocytes, and then this was reverse transcribed to cDNA and amplified via the polymerase chain reaction (PCR) utilizing synthetic oligonucleotides bracketing a portion of the LHRH gene. Following gel electrophoresis a discrete band of the expected size of 375 base pairs was found in the hypothalamus (positive control), and in lymphocytes, but not in the anterior pituitary (negative control). Furthermore, after Southern blotting, a32P-labelled LHRH cDNA, hybridized to the 375 base pair, was amplified in hypothalamus fragments and in lymphocytes, but not in anterior pituitary tissue. These data strongly suggest that LHRH, in addition to being an important neuropeptide, is an immune cell synthesized immunomodulator.

Original languageEnglish (US)
Pages (from-to)1679-1681
Number of pages3
JournalEndocrinology
Volume128
Issue number3
DOIs
StatePublished - Mar 1991

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Gonadotropin-Releasing Hormone
Spleen
Lymphocytes
Base Pairing
Hypothalamus
Complementary DNA
Immunologic Factors
Southern Blotting
Neuropeptides
Oligonucleotides
Electrophoresis
Gels
RNA
Polymerase Chain Reaction
Messenger RNA
Genes

ASJC Scopus subject areas

  • Endocrinology

Cite this

Presence of luteinizing hormone-releasing hormone (Lhrh) mrna in rat spleen lymphocytes. / Azad, Nasrin; Emanuele, Nicholas V.; Halloran, Margaret M.; Tentler, John; Kelley, Mark R.

In: Endocrinology, Vol. 128, No. 3, 03.1991, p. 1679-1681.

Research output: Contribution to journalArticle

Azad, Nasrin ; Emanuele, Nicholas V. ; Halloran, Margaret M. ; Tentler, John ; Kelley, Mark R. / Presence of luteinizing hormone-releasing hormone (Lhrh) mrna in rat spleen lymphocytes. In: Endocrinology. 1991 ; Vol. 128, No. 3. pp. 1679-1681.
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