Presence of precursor ribosome in the ribosomal preparation from chloramphenicol-treated Escherichia coli AB301/I05 (RNase III-)

Debomoy Lahiri, D. P. Burma

Research output: Contribution to journalArticle

Abstract

On sucrose gradient centrifugation, the ribosomal preparation from chloramphenicol-treated32P labelled Escherichia coli AB301/105 (RNase III-) showed the presence of a radioactive peak moving slower than the 70S ribosome; this peak disappeared on treatment with RNase III. The presence of precursor 30S RNA was shown in such preparations by affinity chromatography on a lysine-sepharose 4B column as well as polyacrylamide gel electrophoresis. Dialysis against low Mg concentration followed by sucrose density gradient electrophoresis. Dialysis against dissociation of 70S ribosome into its subunits, did not lead to the dissociation of the precursor ribosome. However, the dissociation took place upon treatment with RNase III. A tentative model of coupled rRNA transcription and ribosome assembly has been presented.

Original languageEnglish (US)
Pages (from-to)379-386
Number of pages8
JournalJournal of Biosciences
Volume2
Issue number4
DOIs
StatePublished - Dec 1980
Externally publishedYes

Fingerprint

Ribonuclease III
Chloramphenicol
chloramphenicol
ribosomes
Ribosomes
Escherichia coli
Dialysis
Electrophoresis
Sucrose
dialysis
Affinity chromatography
Centrifugation
sucrose
Transcription
Sepharose
RNA Precursors
affinity chromatography
Affinity Chromatography
RNA
centrifugation

Keywords

  • precursor
  • ribosomal RNA
  • ribosome assembly
  • Ribosomes
  • RNase III

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Presence of precursor ribosome in the ribosomal preparation from chloramphenicol-treated Escherichia coli AB301/I05 (RNase III-). / Lahiri, Debomoy; Burma, D. P.

In: Journal of Biosciences, Vol. 2, No. 4, 12.1980, p. 379-386.

Research output: Contribution to journalArticle

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