Presentation of abundant endogenous class II DR-restricted antigens by DM-negative B cell lines

Susan Kovats, Phyllis E. Whiteley, Patrick Concannon, Alexander Y. Rudensky, Janice S. Blum

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Peptides derived from endogenous and exogenous antigens compete for binding and presentation via class II molecules. Studies with mutant B cell lines defective in exogenous antigen presentation suggest that HLA-DM molecules facilitate the interaction of foreign peptides and class II molecules. In contrast, presentation of self antigens is not strictly dependent upon HLA-DM, as demonstrated by the ability of these mutant cells to activate T cells specific for endogenous antigens. Two distinct classes of DM-negative cells, T2 cells generated by in vitro mutagenesis and lines derived from bare lymphocyte syndrome (BLS) patients, were able to present epitopes derived from self proteins. Transfection of DM genes into the mutant cells enhanced the presentation of some, but not all, endogenous antigens, suggesting that formation of select endogenous peptide/class II complexes is not dependent upon DM. The efficiency of endogenous antigen presentation in the absence of DM was also dependent on the mutant antigen-presenting cell studied, as the TxB hybrid T2 presented greater amounts of self peptides compared to cells from BLS patients. Thus, additional genes, aside from DM, may regulate the pathway for endogenous antigen presentation.

Original languageEnglish (US)
Pages (from-to)1014-1021
Number of pages8
JournalEuropean Journal of Immunology
Volume27
Issue number4
DOIs
StatePublished - 1997

Keywords

  • Antigen presentation
  • HLA-DM
  • Major histocompatibility complex class II

ASJC Scopus subject areas

  • Immunology

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