Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery

Brandon Crowe, Jeffrey A. Poynter, Mariuxi C. Manukyan, Yue Wang, Benjamin D. Brewster, Jeremy L. Herrmann, Aaron M. Abarbanell, Brent R. Weil, Daniel R. Meldrum

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Cytoprotective growth factors such as vascular endothelial growth factor (VEGF) play important roles in myocardial protection from ischemia/reperfusion (I/R). Accumulating evidence suggests that the hypoxia-inducible factor 1 (HIF-1) pathway is a key regulator of VEGF production in the setting of I/R. The prolyl hydroxylase inhibitor mimosine can increase VEGF production through the HIF-1 pathway. We hypothesized that infusion of preischemic intracoronary mimosine would improve myocardial functional recovery after I/R. Methods: Isolated male rat hearts were subjected to 15 minutes of equilibration, 25 minutes of ischemia, and 40 minutes of reperfusion. Immediately prior to ischemia, ischemic hearts received intracoronary infusions of vehicle or solutions of 0.3, 3, or 30 μM mimosine. Myocardial function was recorded throughout the experiments. Functional data were analyzed with two-way analysis of variance adjusted with the Bonferroni correction. Results: Preischemic myocardial function was equivalent. All hearts had significant reductions in function at the beginning of reperfusion. Hearts treated with 0.3 or 3 μM mimosine infusions exhibited greater recovery of left ventricular developed pressure compared to vehicle. The maximal positive value of the first derivative of pressure (+dP/dt) was greater in hearts treated with 0.3 μM mimosine compared to hearts treated with vehicle. No differences were observed in recovery of end-diastolic pressure or the maximal negative value of the first derivative of pressure (-dP/dt). Conclusion: Preischemic intracoronary mimosine infusion improves myocardial functional recovery after I/R.

Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalSurgery
Volume150
Issue number2
DOIs
StatePublished - Aug 2011

Fingerprint

Mimosine
Vascular Endothelial Growth Factor A
Reperfusion
Hypoxia-Inducible Factor 1
Prolyl-Hydroxylase Inhibitors
Ischemia
Pressure
Ventricular Pressure
Myocardial Ischemia
Intercellular Signaling Peptides and Proteins
Analysis of Variance
Blood Pressure

ASJC Scopus subject areas

  • Surgery

Cite this

Crowe, B., Poynter, J. A., Manukyan, M. C., Wang, Y., Brewster, B. D., Herrmann, J. L., ... Meldrum, D. R. (2011). Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery. Surgery, 150(2), 191-196. https://doi.org/10.1016/j.surg.2011.05.009

Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery. / Crowe, Brandon; Poynter, Jeffrey A.; Manukyan, Mariuxi C.; Wang, Yue; Brewster, Benjamin D.; Herrmann, Jeremy L.; Abarbanell, Aaron M.; Weil, Brent R.; Meldrum, Daniel R.

In: Surgery, Vol. 150, No. 2, 08.2011, p. 191-196.

Research output: Contribution to journalArticle

Crowe, B, Poynter, JA, Manukyan, MC, Wang, Y, Brewster, BD, Herrmann, JL, Abarbanell, AM, Weil, BR & Meldrum, DR 2011, 'Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery', Surgery, vol. 150, no. 2, pp. 191-196. https://doi.org/10.1016/j.surg.2011.05.009
Crowe, Brandon ; Poynter, Jeffrey A. ; Manukyan, Mariuxi C. ; Wang, Yue ; Brewster, Benjamin D. ; Herrmann, Jeremy L. ; Abarbanell, Aaron M. ; Weil, Brent R. ; Meldrum, Daniel R. / Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery. In: Surgery. 2011 ; Vol. 150, No. 2. pp. 191-196.
@article{42bfaad44354417795762fc4d4068da8,
title = "Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery",
abstract = "Background: Cytoprotective growth factors such as vascular endothelial growth factor (VEGF) play important roles in myocardial protection from ischemia/reperfusion (I/R). Accumulating evidence suggests that the hypoxia-inducible factor 1 (HIF-1) pathway is a key regulator of VEGF production in the setting of I/R. The prolyl hydroxylase inhibitor mimosine can increase VEGF production through the HIF-1 pathway. We hypothesized that infusion of preischemic intracoronary mimosine would improve myocardial functional recovery after I/R. Methods: Isolated male rat hearts were subjected to 15 minutes of equilibration, 25 minutes of ischemia, and 40 minutes of reperfusion. Immediately prior to ischemia, ischemic hearts received intracoronary infusions of vehicle or solutions of 0.3, 3, or 30 μM mimosine. Myocardial function was recorded throughout the experiments. Functional data were analyzed with two-way analysis of variance adjusted with the Bonferroni correction. Results: Preischemic myocardial function was equivalent. All hearts had significant reductions in function at the beginning of reperfusion. Hearts treated with 0.3 or 3 μM mimosine infusions exhibited greater recovery of left ventricular developed pressure compared to vehicle. The maximal positive value of the first derivative of pressure (+dP/dt) was greater in hearts treated with 0.3 μM mimosine compared to hearts treated with vehicle. No differences were observed in recovery of end-diastolic pressure or the maximal negative value of the first derivative of pressure (-dP/dt). Conclusion: Preischemic intracoronary mimosine infusion improves myocardial functional recovery after I/R.",
author = "Brandon Crowe and Poynter, {Jeffrey A.} and Manukyan, {Mariuxi C.} and Yue Wang and Brewster, {Benjamin D.} and Herrmann, {Jeremy L.} and Abarbanell, {Aaron M.} and Weil, {Brent R.} and Meldrum, {Daniel R.}",
year = "2011",
month = "8",
doi = "10.1016/j.surg.2011.05.009",
language = "English",
volume = "150",
pages = "191--196",
journal = "Surgery",
issn = "0039-6060",
publisher = "Mosby Inc.",
number = "2",

}

TY - JOUR

T1 - Pretreatment with intracoronary mimosine improves postischemic myocardial functional recovery

AU - Crowe, Brandon

AU - Poynter, Jeffrey A.

AU - Manukyan, Mariuxi C.

AU - Wang, Yue

AU - Brewster, Benjamin D.

AU - Herrmann, Jeremy L.

AU - Abarbanell, Aaron M.

AU - Weil, Brent R.

AU - Meldrum, Daniel R.

PY - 2011/8

Y1 - 2011/8

N2 - Background: Cytoprotective growth factors such as vascular endothelial growth factor (VEGF) play important roles in myocardial protection from ischemia/reperfusion (I/R). Accumulating evidence suggests that the hypoxia-inducible factor 1 (HIF-1) pathway is a key regulator of VEGF production in the setting of I/R. The prolyl hydroxylase inhibitor mimosine can increase VEGF production through the HIF-1 pathway. We hypothesized that infusion of preischemic intracoronary mimosine would improve myocardial functional recovery after I/R. Methods: Isolated male rat hearts were subjected to 15 minutes of equilibration, 25 minutes of ischemia, and 40 minutes of reperfusion. Immediately prior to ischemia, ischemic hearts received intracoronary infusions of vehicle or solutions of 0.3, 3, or 30 μM mimosine. Myocardial function was recorded throughout the experiments. Functional data were analyzed with two-way analysis of variance adjusted with the Bonferroni correction. Results: Preischemic myocardial function was equivalent. All hearts had significant reductions in function at the beginning of reperfusion. Hearts treated with 0.3 or 3 μM mimosine infusions exhibited greater recovery of left ventricular developed pressure compared to vehicle. The maximal positive value of the first derivative of pressure (+dP/dt) was greater in hearts treated with 0.3 μM mimosine compared to hearts treated with vehicle. No differences were observed in recovery of end-diastolic pressure or the maximal negative value of the first derivative of pressure (-dP/dt). Conclusion: Preischemic intracoronary mimosine infusion improves myocardial functional recovery after I/R.

AB - Background: Cytoprotective growth factors such as vascular endothelial growth factor (VEGF) play important roles in myocardial protection from ischemia/reperfusion (I/R). Accumulating evidence suggests that the hypoxia-inducible factor 1 (HIF-1) pathway is a key regulator of VEGF production in the setting of I/R. The prolyl hydroxylase inhibitor mimosine can increase VEGF production through the HIF-1 pathway. We hypothesized that infusion of preischemic intracoronary mimosine would improve myocardial functional recovery after I/R. Methods: Isolated male rat hearts were subjected to 15 minutes of equilibration, 25 minutes of ischemia, and 40 minutes of reperfusion. Immediately prior to ischemia, ischemic hearts received intracoronary infusions of vehicle or solutions of 0.3, 3, or 30 μM mimosine. Myocardial function was recorded throughout the experiments. Functional data were analyzed with two-way analysis of variance adjusted with the Bonferroni correction. Results: Preischemic myocardial function was equivalent. All hearts had significant reductions in function at the beginning of reperfusion. Hearts treated with 0.3 or 3 μM mimosine infusions exhibited greater recovery of left ventricular developed pressure compared to vehicle. The maximal positive value of the first derivative of pressure (+dP/dt) was greater in hearts treated with 0.3 μM mimosine compared to hearts treated with vehicle. No differences were observed in recovery of end-diastolic pressure or the maximal negative value of the first derivative of pressure (-dP/dt). Conclusion: Preischemic intracoronary mimosine infusion improves myocardial functional recovery after I/R.

UR - http://www.scopus.com/inward/record.url?scp=79960905862&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960905862&partnerID=8YFLogxK

U2 - 10.1016/j.surg.2011.05.009

DO - 10.1016/j.surg.2011.05.009

M3 - Article

VL - 150

SP - 191

EP - 196

JO - Surgery

JF - Surgery

SN - 0039-6060

IS - 2

ER -