Pretreatment with low-energy shock waves reduces the renal oxidative stress and inflammation caused by high-energy shock wave lithotripsy

Daniel L. Clark, Bret A. Connors, Rajash Handa, Andrew Evan

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The purpose of this study was to determine if pretreatment of porcine kidneys with low-energy shock waves (SWs) prior to delivery of a clinical dose of 2,000 SWs reduces or prevents shock wave lithotripsy (SWL)-induced acute oxidative stress and inflammation in the treated kidney. Pigs (7-8 weeks old) received 2,000 SWs at 24 kV (120 SW/min) with or without pretreatment with 100 SWs at 12 kV/2 Hz to the lower pole calyx of one kidney using the HM3. Four hours post-treatment, selected samples of renal tissue were frozen for analysis of cytokine, interleukin-6 (IL-6), and stress response protein, heme oxygenase-1 (HO-1). Urine samples were taken before and after treatment for analysis of tumor necrosis factor-α (TNF-α). Treatment with 2,000 SWs with or without pretreatment caused a statistically significant elevation of HO-1 and IL-6 in the renal medulla localized to the focal zone of the lithotripter. However, the increase in HO-1 and IL-6 was significantly reduced using the pretreatment protocol compared to no pretreatment. Urinary excretion of TNF-α increased significantly (p < 0.05) from baseline for pigs receiving 2,000 SWs alone; however, this effect was completely abolished with the pretreatment protocol. We conclude that pretreatment of the kidney with a low dose of low-energy SWs prior to delivery of a clinical dose of SWs reduces, but does not completely prevent, SWL-induced acute renal oxidative stress and inflammation.

Original languageEnglish
Pages (from-to)437-442
Number of pages6
JournalUrological Research
Volume39
Issue number6
DOIs
StatePublished - Dec 2011

Fingerprint

High-Energy Shock Waves
Lithotripsy
Oxidative Stress
Inflammation
Kidney
Heme Oxygenase-1
Interleukin-6
Swine
Tumor Necrosis Factor-alpha
Heat-Shock Proteins
Shock
Therapeutics
Urine
Cytokines

Keywords

  • Heme oxygenase
  • Inflammation
  • Kidney
  • Oxidative stress
  • Shock wave lithotripsy

ASJC Scopus subject areas

  • Urology

Cite this

Pretreatment with low-energy shock waves reduces the renal oxidative stress and inflammation caused by high-energy shock wave lithotripsy. / Clark, Daniel L.; Connors, Bret A.; Handa, Rajash; Evan, Andrew.

In: Urological Research, Vol. 39, No. 6, 12.2011, p. 437-442.

Research output: Contribution to journalArticle

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