Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil

Daniel B. Macieira, Rita de Cássia A A de Menezes, Cristiane B. Damico, Nádia R P Almosny, Heather L. McLane, Joanne Daggy, Joanne B. Messick

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The aim of this study was to determine the prevalence and risk factors for Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (Mhm) infections in domestic cats tested for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) with a commercial enzyme-linked immunosorbent assay (ELISA) kit. Based on serological testing, cats were grouped as i) FIV-positive (n = 25); ii) FeLV-positive (n = 39); iii) FIV/FeLV-positive (n = 8); and iv) FIV/FeLV-negative (n = 77). Complete blood counts were followed by DNA extraction, species-specific polymerase chain reaction (16S rRNA gene) for Mhf and Mhm and Southern blotting for all animals. Mhf DNA was found in 4.0, 2.6, 12.5 and 7.8% of the cats from groups i, ii, iii and iv, respectively, while 32, 5.1, 50 and 5.2% of these animals had an Mhm infection. Cats with FIV (OR = 4.25, P = 0.009) and both FIV and FeLV (OR = 7.56, P = 0.014) were at greater risk of being hemoplasma infected than retroviral-negative cats, mainly due to Mhm infection (OR = 8.59, P = 0.001 and OR = 18.25, P = 0.001, respectively). Among pure-breed cats, FIV-positive status was associated with hemoplasma infection (OR 45.0, P = 0.001).

Original languageEnglish (US)
Pages (from-to)120-129
Number of pages10
JournalJournal of Feline Medicine and Surgery
Volume10
Issue number2
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Feline Leukemia Virus
Feline Immunodeficiency Virus
Feline leukemia virus
Feline immunodeficiency virus
Brazil
Mycoplasma haemominutum
Cats
risk factors
cats
Mycoplasma
Mycoplasma haemofelis
Mycoplasma Infections
infection
Blood Cell Count
DNA
purebreds
Southern Blotting
rRNA Genes
Southern blotting
animals

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil. / Macieira, Daniel B.; de Menezes, Rita de Cássia A A; Damico, Cristiane B.; Almosny, Nádia R P; McLane, Heather L.; Daggy, Joanne; Messick, Joanne B.

In: Journal of Feline Medicine and Surgery, Vol. 10, No. 2, 04.2008, p. 120-129.

Research output: Contribution to journalArticle

Macieira, Daniel B. ; de Menezes, Rita de Cássia A A ; Damico, Cristiane B. ; Almosny, Nádia R P ; McLane, Heather L. ; Daggy, Joanne ; Messick, Joanne B. / Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil. In: Journal of Feline Medicine and Surgery. 2008 ; Vol. 10, No. 2. pp. 120-129.
@article{228641922d6a4e7fb0f2bf7d623cb4c8,
title = "Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil",
abstract = "The aim of this study was to determine the prevalence and risk factors for Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (Mhm) infections in domestic cats tested for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) with a commercial enzyme-linked immunosorbent assay (ELISA) kit. Based on serological testing, cats were grouped as i) FIV-positive (n = 25); ii) FeLV-positive (n = 39); iii) FIV/FeLV-positive (n = 8); and iv) FIV/FeLV-negative (n = 77). Complete blood counts were followed by DNA extraction, species-specific polymerase chain reaction (16S rRNA gene) for Mhf and Mhm and Southern blotting for all animals. Mhf DNA was found in 4.0, 2.6, 12.5 and 7.8{\%} of the cats from groups i, ii, iii and iv, respectively, while 32, 5.1, 50 and 5.2{\%} of these animals had an Mhm infection. Cats with FIV (OR = 4.25, P = 0.009) and both FIV and FeLV (OR = 7.56, P = 0.014) were at greater risk of being hemoplasma infected than retroviral-negative cats, mainly due to Mhm infection (OR = 8.59, P = 0.001 and OR = 18.25, P = 0.001, respectively). Among pure-breed cats, FIV-positive status was associated with hemoplasma infection (OR 45.0, P = 0.001).",
author = "Macieira, {Daniel B.} and {de Menezes}, {Rita de C{\'a}ssia A A} and Damico, {Cristiane B.} and Almosny, {N{\'a}dia R P} and McLane, {Heather L.} and Joanne Daggy and Messick, {Joanne B.}",
year = "2008",
month = "4",
doi = "10.1016/j.jfms.2007.08.002",
language = "English (US)",
volume = "10",
pages = "120--129",
journal = "Journal of Feline Medicine and Surgery",
issn = "1098-612X",
publisher = "W.B. Saunders Ltd",
number = "2",

}

TY - JOUR

T1 - Prevalence and risk factors for hemoplasmas in domestic cats naturally infected with feline immunodeficiency virus and/or feline leukemia virus in Rio de Janeiro - Brazil

AU - Macieira, Daniel B.

AU - de Menezes, Rita de Cássia A A

AU - Damico, Cristiane B.

AU - Almosny, Nádia R P

AU - McLane, Heather L.

AU - Daggy, Joanne

AU - Messick, Joanne B.

PY - 2008/4

Y1 - 2008/4

N2 - The aim of this study was to determine the prevalence and risk factors for Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (Mhm) infections in domestic cats tested for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) with a commercial enzyme-linked immunosorbent assay (ELISA) kit. Based on serological testing, cats were grouped as i) FIV-positive (n = 25); ii) FeLV-positive (n = 39); iii) FIV/FeLV-positive (n = 8); and iv) FIV/FeLV-negative (n = 77). Complete blood counts were followed by DNA extraction, species-specific polymerase chain reaction (16S rRNA gene) for Mhf and Mhm and Southern blotting for all animals. Mhf DNA was found in 4.0, 2.6, 12.5 and 7.8% of the cats from groups i, ii, iii and iv, respectively, while 32, 5.1, 50 and 5.2% of these animals had an Mhm infection. Cats with FIV (OR = 4.25, P = 0.009) and both FIV and FeLV (OR = 7.56, P = 0.014) were at greater risk of being hemoplasma infected than retroviral-negative cats, mainly due to Mhm infection (OR = 8.59, P = 0.001 and OR = 18.25, P = 0.001, respectively). Among pure-breed cats, FIV-positive status was associated with hemoplasma infection (OR 45.0, P = 0.001).

AB - The aim of this study was to determine the prevalence and risk factors for Mycoplasma haemofelis (Mhf) and 'Candidatus Mycoplasma haemominutum' (Mhm) infections in domestic cats tested for feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) with a commercial enzyme-linked immunosorbent assay (ELISA) kit. Based on serological testing, cats were grouped as i) FIV-positive (n = 25); ii) FeLV-positive (n = 39); iii) FIV/FeLV-positive (n = 8); and iv) FIV/FeLV-negative (n = 77). Complete blood counts were followed by DNA extraction, species-specific polymerase chain reaction (16S rRNA gene) for Mhf and Mhm and Southern blotting for all animals. Mhf DNA was found in 4.0, 2.6, 12.5 and 7.8% of the cats from groups i, ii, iii and iv, respectively, while 32, 5.1, 50 and 5.2% of these animals had an Mhm infection. Cats with FIV (OR = 4.25, P = 0.009) and both FIV and FeLV (OR = 7.56, P = 0.014) were at greater risk of being hemoplasma infected than retroviral-negative cats, mainly due to Mhm infection (OR = 8.59, P = 0.001 and OR = 18.25, P = 0.001, respectively). Among pure-breed cats, FIV-positive status was associated with hemoplasma infection (OR 45.0, P = 0.001).

UR - http://www.scopus.com/inward/record.url?scp=42049122822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42049122822&partnerID=8YFLogxK

U2 - 10.1016/j.jfms.2007.08.002

DO - 10.1016/j.jfms.2007.08.002

M3 - Article

C2 - 17905624

AN - SCOPUS:42049122822

VL - 10

SP - 120

EP - 129

JO - Journal of Feline Medicine and Surgery

JF - Journal of Feline Medicine and Surgery

SN - 1098-612X

IS - 2

ER -